TY - GEN

T1 - Imaging of the mouse lung with scanning laser optical tomography (SLOT)

AU - Heidrich, Marko

AU - Kellner, Manuela

AU - Beigel, Rebecca

AU - Lorbeer, Raoul Amadeus

AU - Knudsen, Lars

AU - Heisterkamp, Alexander

AU - Ripken, Tammo

AU - Ochs, Matthias

AU - Kühnel, Mark Philipp

AU - Meyer, Heiko

PY - 2013/3/8

Y1 - 2013/3/8

N2 - New optical techniques have the potential to fill the gap between radiological and microscopic approaches to assess the lung's internal structure. Since its quantitative assessment requires unbiased sampling and measurement principles, imaging of the whole lung with sufficient resolution for visualizing details is important. To address this request, we applied scanning laser optical tomography (SLOT) for the three dimensional imaging of mouse lung ex vivo. SLOT is a highly efficient flourescence and transmission microscopy technique allowing for 3D imaging of specimen of sizes up to several millimeters. Previously fixed lung lobes and whole lungs were optically cleared and subsequently imaged with SLOT while making use of intrinsic contrast mechanisms like absorption and autofluorescence. Imaging of airways, blood vessels and parenchyma is demonstrated. Volumetric SLOT datasets of the lung's internal structure can be analyzed in any preferred planar orientation. Moreover, the sample preparation preserves microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, SLOT is a useful technique to visualize and survey the internal structure of mouse lung at different scales and with various contrast mechanisms. Potential applications of SLOT in lung research are e.g. quantitative phenotype analysis of mouse models of human lung disease in combination with stereological methods.

AB - New optical techniques have the potential to fill the gap between radiological and microscopic approaches to assess the lung's internal structure. Since its quantitative assessment requires unbiased sampling and measurement principles, imaging of the whole lung with sufficient resolution for visualizing details is important. To address this request, we applied scanning laser optical tomography (SLOT) for the three dimensional imaging of mouse lung ex vivo. SLOT is a highly efficient flourescence and transmission microscopy technique allowing for 3D imaging of specimen of sizes up to several millimeters. Previously fixed lung lobes and whole lungs were optically cleared and subsequently imaged with SLOT while making use of intrinsic contrast mechanisms like absorption and autofluorescence. Imaging of airways, blood vessels and parenchyma is demonstrated. Volumetric SLOT datasets of the lung's internal structure can be analyzed in any preferred planar orientation. Moreover, the sample preparation preserves microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, SLOT is a useful technique to visualize and survey the internal structure of mouse lung at different scales and with various contrast mechanisms. Potential applications of SLOT in lung research are e.g. quantitative phenotype analysis of mouse models of human lung disease in combination with stereological methods.

KW - 3D fluorescence microscopy

KW - Acini

KW - Airways

KW - Alveoli

KW - Blood vessels

KW - Optical projection tomography

UR - http://www.scopus.com/inward/record.url?scp=84878208372&partnerID=8YFLogxK

U2 - 10.1117/12.2003955

DO - 10.1117/12.2003955

M3 - Conference contribution

AN - SCOPUS:84878208372

SN - 9780819493347

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Photonic Therapeutics and Diagnostics IX

T2 - Photonic Therapeutics and Diagnostics IX

Y2 - 2 February 2013 through 7 February 2013

ER -