Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Marcus Mandel
  • Linus Holtmann
  • Manuel Raiwa
  • Annika Wunnenberg-Gust
  • Beate Riebe
  • Clemens Walther

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Details

OriginalspracheEnglisch
Aufsatznummer127143
Seitenumfang9
FachzeitschriftJournal of hazardous materials
Jahrgang423
Frühes Online-Datum6 Sept. 2021
PublikationsstatusVeröffentlicht - 5 Feb. 2022

Abstract

In radioecological studies, there is a significant need for understanding the plant uptake of radionuclides on a cellular level. The present work applies mass spectrometry to image the radionuclide distribution within the cellular structures of plants at varying concentrations. In a first step, plants of Daucus carota and Pisum sativum labelled with iodine and rhenium were examined, at concentrations in the range of 10 mM. Cross sections of several plant parts were imaged by secondary ion mass spectrometry (SIMS) after cryogenation in order to preserve cell structure. In a second step, the distribution of 99Tc in the two plant species was determined. For radiological reasons, a concentration three orders of magnitude lower was used, rendering measurements with SIMS impossible. Therefore, resonant laser secondary neutral mass spectrometry (rL-SNMS) was used for the first time to image 99Tc with suppression of molecular isobaric interferences. The measurement of only about 1010 atoms of 99Tc atoms is demonstrated and the distribution of 99Tc within a single epidermal cell is imaged.

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Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. / Mandel, Marcus; Holtmann, Linus; Raiwa, Manuel et al.
in: Journal of hazardous materials, Jahrgang 423, 127143, 05.02.2022.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Mandel, M, Holtmann, L, Raiwa, M, Wunnenberg-Gust, A, Riebe, B & Walther, C 2022, 'Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS', Journal of hazardous materials, Jg. 423, 127143. https://doi.org/10.1016/j.jhazmat.2021.127143
Mandel, M., Holtmann, L., Raiwa, M., Wunnenberg-Gust, A., Riebe, B., & Walther, C. (2022). Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. Journal of hazardous materials, 423, Artikel 127143. https://doi.org/10.1016/j.jhazmat.2021.127143
Mandel M, Holtmann L, Raiwa M, Wunnenberg-Gust A, Riebe B, Walther C. Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. Journal of hazardous materials. 2022 Feb 5;423:127143. Epub 2021 Sep 6. doi: 10.1016/j.jhazmat.2021.127143
Mandel, Marcus ; Holtmann, Linus ; Raiwa, Manuel et al. / Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. in: Journal of hazardous materials. 2022 ; Jahrgang 423.
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abstract = "In radioecological studies, there is a significant need for understanding the plant uptake of radionuclides on a cellular level. The present work applies mass spectrometry to image the radionuclide distribution within the cellular structures of plants at varying concentrations. In a first step, plants of Daucus carota and Pisum sativum labelled with iodine and rhenium were examined, at concentrations in the range of 10 mM. Cross sections of several plant parts were imaged by secondary ion mass spectrometry (SIMS) after cryogenation in order to preserve cell structure. In a second step, the distribution of 99Tc in the two plant species was determined. For radiological reasons, a concentration three orders of magnitude lower was used, rendering measurements with SIMS impossible. Therefore, resonant laser secondary neutral mass spectrometry (rL-SNMS) was used for the first time to image 99Tc with suppression of molecular isobaric interferences. The measurement of only about 1010 atoms of 99Tc atoms is demonstrated and the distribution of 99Tc within a single epidermal cell is imaged.",
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AU - Mandel, Marcus

AU - Holtmann, Linus

AU - Raiwa, Manuel

AU - Wunnenberg-Gust, Annika

AU - Riebe, Beate

AU - Walther, Clemens

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