Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Jens Hellmer
  • Ralf Pätzold
  • Carsten Zeilinger

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Details

OriginalspracheEnglisch
Seiten (von - bis)245-9
Seitenumfang5
FachzeitschriftFEBS letters
Jahrgang527
Ausgabenummer1-3
PublikationsstatusVeröffentlicht - 11 Sept. 2002

Abstract

The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.

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Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii. / Hellmer, Jens; Pätzold, Ralf; Zeilinger, Carsten.
in: FEBS letters, Jahrgang 527, Nr. 1-3, 11.09.2002, S. 245-9.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Hellmer J, Pätzold R, Zeilinger C. Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii. FEBS letters. 2002 Sep 11;527(1-3):245-9. doi: 10.1016/s0014-5793(02)03244-1
Hellmer, Jens ; Pätzold, Ralf ; Zeilinger, Carsten. / Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii. in: FEBS letters. 2002 ; Jahrgang 527, Nr. 1-3. S. 245-9.
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title = "Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii",
abstract = "The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.",
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TY - JOUR

T1 - Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii

AU - Hellmer, Jens

AU - Pätzold, Ralf

AU - Zeilinger, Carsten

PY - 2002/9/11

Y1 - 2002/9/11

N2 - The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.

AB - The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.

KW - Amino Acid Sequence

KW - Archaeal Proteins/genetics

KW - Bacterial Proteins

KW - Biological Transport

KW - Cloning, Molecular

KW - Escherichia coli/genetics

KW - Escherichia coli Proteins

KW - Genetic Complementation Test

KW - Hydrogen-Ion Concentration

KW - Membrane Proteins/genetics

KW - Methanococcus/genetics

KW - Molecular Sequence Data

KW - Multigene Family

KW - Mutation

KW - Phylogeny

KW - Sequence Homology, Amino Acid

KW - Sodium/metabolism

KW - Sodium-Hydrogen Exchangers/genetics

U2 - 10.1016/s0014-5793(02)03244-1

DO - 10.1016/s0014-5793(02)03244-1

M3 - Article

C2 - 12220668

VL - 527

SP - 245

EP - 249

JO - FEBS letters

JF - FEBS letters

SN - 0014-5793

IS - 1-3

ER -