TY - JOUR

T1 - Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

AU - Nikolits, Ilias

AU - Radwan, Sara

AU - Liebner, Falk

AU - Dietrich, Wolf

AU - Egger, Dominik

AU - Chariyev-Prinz, Farhad

AU - Kasper, Cornelia

N1 - Funding Information: This project was supported by the Doctoral School “Biomaterials and Biointerfaces (BioMatInt)” of the University of Natural Resources and Life Sciences BOKU Vienna.

PY - 2023/2/20

Y1 - 2023/2/20

N2 - Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6-tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2-0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.

AB - Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6-tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2-0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.

KW - 3D cultivation

KW - cellulose

KW - hydrogel

KW - in vitro culture

KW - mesenchymal stem cells

UR - http://www.scopus.com/inward/record.url?scp=85147823505&partnerID=8YFLogxK

U2 - 10.1021/acsabm.2c00854

DO - 10.1021/acsabm.2c00854

M3 - Article

C2 - 36745634

AN - SCOPUS:85147823505

VL - 6

SP - 543

EP - 551

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

IS - 2

ER -