Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Dmitrii Iudin
  • Marina Vasilieva
  • Elena Knyazeva
  • Viktor Korzhikov-Vlakh
  • Elena Demyanova
  • Antonina Lavrentieva
  • Yury Skorik
  • Evgenia Korzhikova-Vlakh

Organisationseinheiten

Externe Organisationen

  • Staatliche Universität Sankt Petersburg
  • Russian Academy of Sciences (RAS)
  • State Research Institute of Highly Pure Biopreparations
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer2771
FachzeitschriftInternational Journal of Molecular Sciences
Jahrgang23
Ausgabenummer5
PublikationsstatusVeröffentlicht - 2 März 2022

Abstract

The growing number of drug-resistant pathogenic bacteria poses a global threat to human health. For this reason, the search for ways to enhance the antibacterial activity of existing antibiotics is now an urgent medical task. The aim of this study was to develop novel delivery systems for polymyxins to improve their antimicrobial properties against various infections. For this, hybrid core–shell nanoparticles, consisting of silver core and a poly(glutamic acid) shell capable of poly-myxin binding, were developed and carefully investigated. Characterization of the hybrid nanopar-ticles revealed a hydrodynamic diameter of approximately 100 nm and a negative electrokinetic potential. The nanoparticles demonstrated a lack of cytotoxicity, a low uptake by macrophages, and their own antimicrobial activity. Drug loading and loading efficacy were determined for both pol-ymyxin B and E, and the maximal loaded value with an appropriate size of the delivery systems was 450 µg/mg of nanoparticles. Composite materials based on agarose hydrogel were prepared, containing both the loaded hybrid systems and free antibiotics. The features of polymyxin release from the hybrid nanoparticles and the composite materials were studied, and the mechanisms of release were analyzed using different theoretical models. The antibacterial activity against Pseudo-monas aeruginosa was evaluated for both the polymyxin hybrid and the composite delivery systems. All tested samples inhibited bacterial growth. The minimal inhibitory concentrations of the poly-myxin B hybrid delivery system demonstrated a synergistic effect when compared with either the antibiotic or the silver nanoparticles alone.

ASJC Scopus Sachgebiete

Ziele für nachhaltige Entwicklung

Zitieren

Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics. / Iudin, Dmitrii; Vasilieva, Marina; Knyazeva, Elena et al.
in: International Journal of Molecular Sciences, Jahrgang 23, Nr. 5, 2771, 02.03.2022.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Iudin, D, Vasilieva, M, Knyazeva, E, Korzhikov-Vlakh, V, Demyanova, E, Lavrentieva, A, Skorik, Y & Korzhikova-Vlakh, E 2022, 'Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics', International Journal of Molecular Sciences, Jg. 23, Nr. 5, 2771. https://doi.org/10.3390/ijms23052771
Iudin, D., Vasilieva, M., Knyazeva, E., Korzhikov-Vlakh, V., Demyanova, E., Lavrentieva, A., Skorik, Y., & Korzhikova-Vlakh, E. (2022). Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics. International Journal of Molecular Sciences, 23(5), Artikel 2771. https://doi.org/10.3390/ijms23052771
Iudin D, Vasilieva M, Knyazeva E, Korzhikov-Vlakh V, Demyanova E, Lavrentieva A et al. Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics. International Journal of Molecular Sciences. 2022 Mär 2;23(5):2771. doi: 10.3390/ijms23052771
Iudin, Dmitrii ; Vasilieva, Marina ; Knyazeva, Elena et al. / Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics. in: International Journal of Molecular Sciences. 2022 ; Jahrgang 23, Nr. 5.
Download
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abstract = "The growing number of drug-resistant pathogenic bacteria poses a global threat to human health. For this reason, the search for ways to enhance the antibacterial activity of existing antibiotics is now an urgent medical task. The aim of this study was to develop novel delivery systems for polymyxins to improve their antimicrobial properties against various infections. For this, hybrid core–shell nanoparticles, consisting of silver core and a poly(glutamic acid) shell capable of poly-myxin binding, were developed and carefully investigated. Characterization of the hybrid nanopar-ticles revealed a hydrodynamic diameter of approximately 100 nm and a negative electrokinetic potential. The nanoparticles demonstrated a lack of cytotoxicity, a low uptake by macrophages, and their own antimicrobial activity. Drug loading and loading efficacy were determined for both pol-ymyxin B and E, and the maximal loaded value with an appropriate size of the delivery systems was 450 µg/mg of nanoparticles. Composite materials based on agarose hydrogel were prepared, containing both the loaded hybrid systems and free antibiotics. The features of polymyxin release from the hybrid nanoparticles and the composite materials were studied, and the mechanisms of release were analyzed using different theoretical models. The antibacterial activity against Pseudo-monas aeruginosa was evaluated for both the polymyxin hybrid and the composite delivery systems. All tested samples inhibited bacterial growth. The minimal inhibitory concentrations of the poly-myxin B hybrid delivery system demonstrated a synergistic effect when compared with either the antibiotic or the silver nanoparticles alone.",
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T1 - Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics

AU - Iudin, Dmitrii

AU - Vasilieva, Marina

AU - Knyazeva, Elena

AU - Korzhikov-Vlakh, Viktor

AU - Demyanova, Elena

AU - Lavrentieva, Antonina

AU - Skorik, Yury

AU - Korzhikova-Vlakh, Evgenia

N1 - Funding Information: This research was funded by the Russian Science Foundation (project no. 19-73-20157).

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