TY - JOUR

T1 - Holographic optogenetic stimulation with calcium imaging as an all optical tool for cardiac electrophysiology

AU - Junge, Sebastian

AU - Schmieder, Felix

AU - Sasse, Philipp

AU - Czarske, Jürgen

AU - Torres-Mapa, Maria Leilani

AU - Heisterkamp, Alexander

N1 - Funding Information: We acknowledge the support from the Federal Ministry of Education and Research, Germany (13N14085, Alexander Heisterkamp and Philipp Sasse). This study was partly supported by the German Research Foundation, Germany Clusters of Excellence: REBIRTH (EXC 62) and Hearing4all (EXC 2177) (Alexander Heisterkamp), Physics of Life (2068) (Jürgen Czarske) and German Research Foundation, project CZ55/39 (Jürgen Czarske). Maria Leilani Torres‐Mapa acknowledges the support of Caroline Herschel Program from the Hochschulbüro für Chancenvielfalt, Leibniz University Hannover. Open Access funding enabled and organized by Projekt DEAL.

PY - 2022/7

Y1 - 2022/7

N2 - All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin-2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal-630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium.

AB - All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin-2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal-630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium.

KW - calcium imaging

KW - cardiac electrophysiology

KW - channelrhodopsin

KW - optogenetics

KW - spatial light modulator

KW - wavefront shaping

UR - http://www.scopus.com/inward/record.url?scp=85129169794&partnerID=8YFLogxK

U2 - 10.1002/jbio.202100352

DO - 10.1002/jbio.202100352

M3 - Article

C2 - 35397155

AN - SCOPUS:85129169794

VL - 15

JO - Journal of biophotonics

JF - Journal of biophotonics

SN - 1864-063X

IS - 7

M1 - e202100352

ER -