Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Raoul Amadeus Lorbeer
  • Marko Heidrich
  • Christina Lorbeer
  • Diego Fernando Ramírez Ojeda
  • Gerd Bicker
  • Heiko Meyer
  • Alexander Heisterkamp

Organisationseinheiten

Externe Organisationen

  • Laser Zentrum Hannover e.V. (LZH)
  • Stiftung Tierärztliche Hochschule Hannover
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Details

OriginalspracheEnglisch
Seiten (von - bis)5419-5430
Seitenumfang12
FachzeitschriftOptics Express
Jahrgang19
Ausgabenummer6
PublikationsstatusVeröffentlicht - 14 März 2011

Abstract

Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD-or CMOS-camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold.

ASJC Scopus Sachgebiete

Zitieren

Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph. / Lorbeer, Raoul Amadeus; Heidrich, Marko; Lorbeer, Christina et al.
in: Optics Express, Jahrgang 19, Nr. 6, 14.03.2011, S. 5419-5430.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Lorbeer, RA, Heidrich, M, Lorbeer, C, Ojeda, DFR, Bicker, G, Meyer, H & Heisterkamp, A 2011, 'Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph', Optics Express, Jg. 19, Nr. 6, S. 5419-5430. https://doi.org/10.1364/OE.19.005419
Lorbeer, R. A., Heidrich, M., Lorbeer, C., Ojeda, D. F. R., Bicker, G., Meyer, H., & Heisterkamp, A. (2011). Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph. Optics Express, 19(6), 5419-5430. https://doi.org/10.1364/OE.19.005419
Lorbeer RA, Heidrich M, Lorbeer C, Ojeda DFR, Bicker G, Meyer H et al. Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph. Optics Express. 2011 Mär 14;19(6):5419-5430. doi: 10.1364/OE.19.005419
Lorbeer, Raoul Amadeus ; Heidrich, Marko ; Lorbeer, Christina et al. / Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph. in: Optics Express. 2011 ; Jahrgang 19, Nr. 6. S. 5419-5430.
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AU - Lorbeer, Christina

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