Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • B. S. Shavkuta
  • M. Y. Gerasimov
  • N. V. Minaev
  • D. S. Kuznetsova
  • V. V. Dudenkova
  • I. A. Mushkova
  • B. E. Malyugin
  • S. L. Kotova
  • P. S. Timashev
  • S. V. Kostenev
  • B. N. Chichkov
  • V. N. Bagratashvili

Externe Organisationen

  • Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics” of the Russian Academy of Sciences
  • S. Fyodorov Eye Microsurgery State Institution
  • Nizhny Novgorod State Medical Acad.
  • RAS - N.N.Semenov Institute of Chemical Physics
  • Sechenov First Moscow State Medical University
  • Laser Zentrum Hannover e.V. (LZH)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer015602
FachzeitschriftLaser physics letters
Jahrgang15
Ausgabenummer1
PublikationsstatusVeröffentlicht - 13 Dez. 2017
Extern publiziertJa

Abstract

A two-photon laser femtosecond crosslinking process at the wavelength of 525 nm was studied in a human donor cornea in the presence of riboflavin using two-photon optical microscopy and nanoindentation. It was shown that such an approach results in efficient crosslinking of the corneal collagen and a significant (three-fold) increase in the Young's modulus of the corneal structure. Application of a femtosecond laser with the wavelength of 525 nm allows a drastic enhancement of efficiency in the presence of riboflavin on human corneas and a 50-fold reduction of the laser treatment duration in comparison with the use of a femtosecond laser with the wavelength of 760 nm. We relate this effect to a significant growth in the coefficient of two-photon absorption due to the laser wavelength falling within the edge of the photoinitiator (riboflavin) absorption band. Our studies on a donor human cornea demonstrate the good potential for the clinical application of a femtosecond laser with the wavelength of 525 nm for increasing the cornea rigidity using the two-photon laser femtosecond crosslinking technique.

ASJC Scopus Sachgebiete

Zitieren

Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. / Shavkuta, B. S.; Gerasimov, M. Y.; Minaev, N. V. et al.
in: Laser physics letters, Jahrgang 15, Nr. 1, 015602, 13.12.2017.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Shavkuta, BS, Gerasimov, MY, Minaev, NV, Kuznetsova, DS, Dudenkova, VV, Mushkova, IA, Malyugin, BE, Kotova, SL, Timashev, PS, Kostenev, SV, Chichkov, BN & Bagratashvili, VN 2017, 'Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea', Laser physics letters, Jg. 15, Nr. 1, 015602. https://doi.org/10.1088/1612-202x/aa963b
Shavkuta, B. S., Gerasimov, M. Y., Minaev, N. V., Kuznetsova, D. S., Dudenkova, V. V., Mushkova, I. A., Malyugin, B. E., Kotova, S. L., Timashev, P. S., Kostenev, S. V., Chichkov, B. N., & Bagratashvili, V. N. (2017). Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. Laser physics letters, 15(1), Artikel 015602. https://doi.org/10.1088/1612-202x/aa963b
Shavkuta BS, Gerasimov MY, Minaev NV, Kuznetsova DS, Dudenkova VV, Mushkova IA et al. Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. Laser physics letters. 2017 Dez 13;15(1):015602. doi: 10.1088/1612-202x/aa963b
Shavkuta, B. S. ; Gerasimov, M. Y. ; Minaev, N. V. et al. / Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. in: Laser physics letters. 2017 ; Jahrgang 15, Nr. 1.
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abstract = "A two-photon laser femtosecond crosslinking process at the wavelength of 525 nm was studied in a human donor cornea in the presence of riboflavin using two-photon optical microscopy and nanoindentation. It was shown that such an approach results in efficient crosslinking of the corneal collagen and a significant (three-fold) increase in the Young's modulus of the corneal structure. Application of a femtosecond laser with the wavelength of 525 nm allows a drastic enhancement of efficiency in the presence of riboflavin on human corneas and a 50-fold reduction of the laser treatment duration in comparison with the use of a femtosecond laser with the wavelength of 760 nm. We relate this effect to a significant growth in the coefficient of two-photon absorption due to the laser wavelength falling within the edge of the photoinitiator (riboflavin) absorption band. Our studies on a donor human cornea demonstrate the good potential for the clinical application of a femtosecond laser with the wavelength of 525 nm for increasing the cornea rigidity using the two-photon laser femtosecond crosslinking technique.",
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AU - Shavkuta, B. S.

AU - Gerasimov, M. Y.

AU - Minaev, N. V.

AU - Kuznetsova, D. S.

AU - Dudenkova, V. V.

AU - Mushkova, I. A.

AU - Malyugin, B. E.

AU - Kotova, S. L.

AU - Timashev, P. S.

AU - Kostenev, S. V.

AU - Chichkov, B. N.

AU - Bagratashvili, V. N.

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PY - 2017/12/13

Y1 - 2017/12/13

N2 - A two-photon laser femtosecond crosslinking process at the wavelength of 525 nm was studied in a human donor cornea in the presence of riboflavin using two-photon optical microscopy and nanoindentation. It was shown that such an approach results in efficient crosslinking of the corneal collagen and a significant (three-fold) increase in the Young's modulus of the corneal structure. Application of a femtosecond laser with the wavelength of 525 nm allows a drastic enhancement of efficiency in the presence of riboflavin on human corneas and a 50-fold reduction of the laser treatment duration in comparison with the use of a femtosecond laser with the wavelength of 760 nm. We relate this effect to a significant growth in the coefficient of two-photon absorption due to the laser wavelength falling within the edge of the photoinitiator (riboflavin) absorption band. Our studies on a donor human cornea demonstrate the good potential for the clinical application of a femtosecond laser with the wavelength of 525 nm for increasing the cornea rigidity using the two-photon laser femtosecond crosslinking technique.

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