TY - JOUR

T1 - Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization

AU - Shenouda, Mary L.

AU - Ambilika, Maria

AU - Skellam, Elizabeth

AU - Cox, Russell J.

N1 - Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG (CO 1328/2-1, INST 187/621-1, INST 187/686-1). The APC was funded by the Open Access Fund of the Leibniz Universität Hannover.

PY - 2022/4

Y1 - 2022/4

N2 - Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.

AB - Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.

KW - heterologous expression

KW - microbial cell factory

KW - PKS

KW - PKS-NRPS

KW - Trichoderma reesei

KW - waste valorization

UR - http://www.scopus.com/inward/record.url?scp=85128351684&partnerID=8YFLogxK

U2 - 10.3390/jof8040355

DO - 10.3390/jof8040355

M3 - Article

AN - SCOPUS:85128351684

VL - 8

JO - Journal of Fungi

JF - Journal of Fungi

IS - 4

M1 - 355

ER -