Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 51 |
| Seitenumfang | 15 |
| Fachzeitschrift | Cell and Bioscience |
| Jahrgang | 12 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 2 Mai 2022 |
| Extern publiziert | Ja |
Abstract
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects previously attributed to cell-based therapies have been recognized to be actually mediated by EVs derived from the respective cells, suggesting the administration of purified EVs instead of living cells for cell-based therapies. In this review, we focus on the heterogeneity of EVs derived from mesenchymal stem/stromal cells (MSC) and summarize upstream process parameters that crucially affect the resulting therapeutic properties and biological functions. Hereby, we discuss the effects of the cell source, medium composition, 3D culture, bioreactor culture and hypoxia. Furthermore, aspects of the isolation and storage strategies influences EVs are described. Conclusively, optimization of upstream process parameters should focus on controlling MSC-derived EV heterogeneity for specific therapeutic applications. Graphical Abstract: [Figure not available: see fulltext.]
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Allgemeine Biochemie, Genetik und Molekularbiologie
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in: Cell and Bioscience, Jahrgang 12, Nr. 1, 51, 02.05.2022.
Publikation: Beitrag in Fachzeitschrift › Übersichtsarbeit › Forschung › Peer-Review
}
TY - JOUR
T1 - Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions
AU - Almeria, Ciarra
AU - Kreß, Sebastian
AU - Weber, Viktoria
AU - Egger, Dominik
AU - Kasper, Cornelia
N1 - Funding Information: No applicable.
PY - 2022/5/2
Y1 - 2022/5/2
N2 - Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects previously attributed to cell-based therapies have been recognized to be actually mediated by EVs derived from the respective cells, suggesting the administration of purified EVs instead of living cells for cell-based therapies. In this review, we focus on the heterogeneity of EVs derived from mesenchymal stem/stromal cells (MSC) and summarize upstream process parameters that crucially affect the resulting therapeutic properties and biological functions. Hereby, we discuss the effects of the cell source, medium composition, 3D culture, bioreactor culture and hypoxia. Furthermore, aspects of the isolation and storage strategies influences EVs are described. Conclusively, optimization of upstream process parameters should focus on controlling MSC-derived EV heterogeneity for specific therapeutic applications. Graphical Abstract: [Figure not available: see fulltext.]
AB - Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects previously attributed to cell-based therapies have been recognized to be actually mediated by EVs derived from the respective cells, suggesting the administration of purified EVs instead of living cells for cell-based therapies. In this review, we focus on the heterogeneity of EVs derived from mesenchymal stem/stromal cells (MSC) and summarize upstream process parameters that crucially affect the resulting therapeutic properties and biological functions. Hereby, we discuss the effects of the cell source, medium composition, 3D culture, bioreactor culture and hypoxia. Furthermore, aspects of the isolation and storage strategies influences EVs are described. Conclusively, optimization of upstream process parameters should focus on controlling MSC-derived EV heterogeneity for specific therapeutic applications. Graphical Abstract: [Figure not available: see fulltext.]
KW - Cell Culture Conditions
KW - Extracellular vesicles
KW - Mesenchymal Stem Cells
KW - Regenerative Medicine
KW - Scalability
UR - http://www.scopus.com/inward/record.url?scp=85129294378&partnerID=8YFLogxK
U2 - 10.1186/s13578-022-00786-7
DO - 10.1186/s13578-022-00786-7
M3 - Review article
AN - SCOPUS:85129294378
VL - 12
JO - Cell and Bioscience
JF - Cell and Bioscience
SN - 2045-3701
IS - 1
M1 - 51
ER -