Loading [MathJax]/extensions/tex2jax.js

Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Anke Becker
  • Hélène Bergès
  • Elizaveta Krol
  • Claude Bruand
  • Helge Küster

Externe Organisationen

  • Universität Bielefeld
  • Institut national de recherche pour l’agriculture, l’alimentation et l’environnement (INRAE)
Plum Print visual indicator of research metrics
  • Citations
    • Citation Indexes: 217
  • Captures
    • Readers: 153
  • Mentions
    • Blog Mentions: 1
    • References: 1
see details

Details

OriginalspracheEnglisch
Seiten (von - bis)292-303
Seitenumfang12
FachzeitschriftMolecular Plant-Microbe Interactions
Jahrgang17
Ausgabenummer3
PublikationsstatusVeröffentlicht - März 2004
Extern publiziertJa

Abstract

Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.

ASJC Scopus Sachgebiete

Zitieren

Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. / Becker, Anke; Bergès, Hélène; Krol, Elizaveta et al.
in: Molecular Plant-Microbe Interactions, Jahrgang 17, Nr. 3, 03.2004, S. 292-303.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Becker, A, Bergès, H, Krol, E, Bruand, C, Rüberg, S, Capela, D, Lauber, E, Meilhoc, E, Ampe, F, De Bruijn, FJ, Fourment, J, Francez-Charlot, A, Kahn, D, Küster, H, Liebe, C, Pühler, A, Weidner, S & Batut, J 2004, 'Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions', Molecular Plant-Microbe Interactions, Jg. 17, Nr. 3, S. 292-303. https://doi.org/10.1094/MPMI.2004.17.3.292
Becker, A., Bergès, H., Krol, E., Bruand, C., Rüberg, S., Capela, D., Lauber, E., Meilhoc, E., Ampe, F., De Bruijn, F. J., Fourment, J., Francez-Charlot, A., Kahn, D., Küster, H., Liebe, C., Pühler, A., Weidner, S., & Batut, J. (2004). Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Molecular Plant-Microbe Interactions, 17(3), 292-303. https://doi.org/10.1094/MPMI.2004.17.3.292
Becker A, Bergès H, Krol E, Bruand C, Rüberg S, Capela D et al. Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Molecular Plant-Microbe Interactions. 2004 Mär;17(3):292-303. doi: 10.1094/MPMI.2004.17.3.292
Becker, Anke ; Bergès, Hélène ; Krol, Elizaveta et al. / Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. in: Molecular Plant-Microbe Interactions. 2004 ; Jahrgang 17, Nr. 3. S. 292-303.
Download
@article{f812046dce6f404480dd915276eb0153,
title = "Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions",
abstract = "Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.",
keywords = "Macroarray, Root nodule",
author = "Anke Becker and H{\'e}l{\`e}ne Berg{\`e}s and Elizaveta Krol and Claude Bruand and Silvia R{\"u}berg and Delphine Capela and Emmanuelle Lauber and Eliane Meilhoc and Fr{\'e}d{\'e}ric Ampe and {De Bruijn}, {Frans J.} and Jo{\"e}lle Fourment and Anne Francez-Charlot and Daniel Kahn and Helge K{\"u}ster and Carine Liebe and Alfred P{\"u}hler and Stefan Weidner and Jacques Batut",
year = "2004",
month = mar,
doi = "10.1094/MPMI.2004.17.3.292",
language = "English",
volume = "17",
pages = "292--303",
journal = "Molecular Plant-Microbe Interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "3",

}

Download

TY - JOUR

T1 - Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions

AU - Becker, Anke

AU - Bergès, Hélène

AU - Krol, Elizaveta

AU - Bruand, Claude

AU - Rüberg, Silvia

AU - Capela, Delphine

AU - Lauber, Emmanuelle

AU - Meilhoc, Eliane

AU - Ampe, Frédéric

AU - De Bruijn, Frans J.

AU - Fourment, Joëlle

AU - Francez-Charlot, Anne

AU - Kahn, Daniel

AU - Küster, Helge

AU - Liebe, Carine

AU - Pühler, Alfred

AU - Weidner, Stefan

AU - Batut, Jacques

PY - 2004/3

Y1 - 2004/3

N2 - Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.

AB - Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.

KW - Macroarray

KW - Root nodule

UR - http://www.scopus.com/inward/record.url?scp=12144290956&partnerID=8YFLogxK

U2 - 10.1094/MPMI.2004.17.3.292

DO - 10.1094/MPMI.2004.17.3.292

M3 - Article

C2 - 15000396

AN - SCOPUS:12144290956

VL - 17

SP - 292

EP - 303

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

SN - 0894-0282

IS - 3

ER -

Von denselben Autoren