Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 169-178 |
| Seitenumfang | 10 |
| Fachzeitschrift | Plant science |
| Jahrgang | 155 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 29 Juni 2000 |
| Extern publiziert | Ja |
Abstract
A full-length cDNA encoding the broad bean (Vicia faba L.) early nodulin VfENOD5 was isolated from a nodule cDNA library. In addition to the ENOD5 homologues from other legumes the derived VfENOD5 amino acid sequence also displayed homologies to the phytocyanin-related nodulins GmENOD55-2, MtENOD16, and MtENOD20. A close inspection of the ENOD5 proteins from broad bean, pea and vetch indicated that all these nodulins possess a putative C- terminal GPI-anchor signal sequence. This novel finding supports the hypothesis that ENOD5 is an arabinogalactan protein. Tissues print hybridizations revealed that the broad bean ENOD5 gene was not only expressed in the central tissues of root nodules. In contrast to other legumes hybridizing transcripts were also be detected in a narrow zone within the peripheral nodule tissues. Sequences analysis of a genomic clone indicated the presence of a single intron interrupting the VfENOD5 coding region at a position precisely corresponding to the MtENOD16 and MtENOD20 introns. (C) 2000 Elsevier Ireland Ltd.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Plant science, Jahrgang 155, Nr. 2, 29.06.2000, S. 169-178.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Genomic organization and expression properties of the VfENOD5 gene from broad bean (Vicia faba L.)
AU - Frühling, Martin
AU - Hohnjec, Natalija
AU - Schröder, Gerald
AU - Küster, Helge
AU - Pühler, Alfred
AU - Perlick, Andreas M.
PY - 2000/6/29
Y1 - 2000/6/29
N2 - A full-length cDNA encoding the broad bean (Vicia faba L.) early nodulin VfENOD5 was isolated from a nodule cDNA library. In addition to the ENOD5 homologues from other legumes the derived VfENOD5 amino acid sequence also displayed homologies to the phytocyanin-related nodulins GmENOD55-2, MtENOD16, and MtENOD20. A close inspection of the ENOD5 proteins from broad bean, pea and vetch indicated that all these nodulins possess a putative C- terminal GPI-anchor signal sequence. This novel finding supports the hypothesis that ENOD5 is an arabinogalactan protein. Tissues print hybridizations revealed that the broad bean ENOD5 gene was not only expressed in the central tissues of root nodules. In contrast to other legumes hybridizing transcripts were also be detected in a narrow zone within the peripheral nodule tissues. Sequences analysis of a genomic clone indicated the presence of a single intron interrupting the VfENOD5 coding region at a position precisely corresponding to the MtENOD16 and MtENOD20 introns. (C) 2000 Elsevier Ireland Ltd.
AB - A full-length cDNA encoding the broad bean (Vicia faba L.) early nodulin VfENOD5 was isolated from a nodule cDNA library. In addition to the ENOD5 homologues from other legumes the derived VfENOD5 amino acid sequence also displayed homologies to the phytocyanin-related nodulins GmENOD55-2, MtENOD16, and MtENOD20. A close inspection of the ENOD5 proteins from broad bean, pea and vetch indicated that all these nodulins possess a putative C- terminal GPI-anchor signal sequence. This novel finding supports the hypothesis that ENOD5 is an arabinogalactan protein. Tissues print hybridizations revealed that the broad bean ENOD5 gene was not only expressed in the central tissues of root nodules. In contrast to other legumes hybridizing transcripts were also be detected in a narrow zone within the peripheral nodule tissues. Sequences analysis of a genomic clone indicated the presence of a single intron interrupting the VfENOD5 coding region at a position precisely corresponding to the MtENOD16 and MtENOD20 introns. (C) 2000 Elsevier Ireland Ltd.
KW - VfENOD5 gene
KW - Vicia faba L.
UR - http://www.scopus.com/inward/record.url?scp=0343962200&partnerID=8YFLogxK
U2 - 10.1016/S0168-9452(00)00216-8
DO - 10.1016/S0168-9452(00)00216-8
M3 - Article
AN - SCOPUS:0343962200
VL - 155
SP - 169
EP - 178
JO - Plant science
JF - Plant science
SN - 0168-9452
IS - 2
ER -