Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 2214-2219 |
| Seitenumfang | 6 |
| Fachzeitschrift | Journal of proteomics |
| Jahrgang | 74 |
| Ausgabenummer | 10 |
| Publikationsstatus | Veröffentlicht - 25 Juni 2011 |
Abstract
Protein separation by two-dimensional gel electrophoresis is of central importance for proteomics. Upon combination with systematic protein identifications by mass spectrometry, large data sets are routinely generated in several proteome laboratories which can be used as "reference maps" for future analyses of analogous biochemical fractions. Here we present GelMap, a novel software tool for the building presentation and evaluation of proteomic reference maps. Variable frames are introduced in order to group proteins into functional categories on three levels or into categories according to differential abundance during comparative proteome analyses. The software is easy to handle as it only requires uploading two digital files to a web site. An additional file including detailed information on all proteins can be combined with the primary map. Two different gel-based projects are presented to illustrate the capacity of GelMap for proteome annotation and evaluation.
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in: Journal of proteomics, Jahrgang 74, Nr. 10, 25.06.2011, S. 2214-2219.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - GelMap
T2 - A novel software tool for building and presenting proteome reference maps
AU - Rode, Christina
AU - Senkler, Michael
AU - Klodmann, Jennifer
AU - Winkelmann, Traud
AU - Braun, Hans Peter
N1 - Funding information: The authors would like to thank Dr. Frank Colditz for critical discussion, Dr. Holger Eubel for critical proof reading the manuscript and the DFG (German Research Foundation , project number WI2002/3-1 ) for financial support.
PY - 2011/6/25
Y1 - 2011/6/25
N2 - Protein separation by two-dimensional gel electrophoresis is of central importance for proteomics. Upon combination with systematic protein identifications by mass spectrometry, large data sets are routinely generated in several proteome laboratories which can be used as "reference maps" for future analyses of analogous biochemical fractions. Here we present GelMap, a novel software tool for the building presentation and evaluation of proteomic reference maps. Variable frames are introduced in order to group proteins into functional categories on three levels or into categories according to differential abundance during comparative proteome analyses. The software is easy to handle as it only requires uploading two digital files to a web site. An additional file including detailed information on all proteins can be combined with the primary map. Two different gel-based projects are presented to illustrate the capacity of GelMap for proteome annotation and evaluation.
AB - Protein separation by two-dimensional gel electrophoresis is of central importance for proteomics. Upon combination with systematic protein identifications by mass spectrometry, large data sets are routinely generated in several proteome laboratories which can be used as "reference maps" for future analyses of analogous biochemical fractions. Here we present GelMap, a novel software tool for the building presentation and evaluation of proteomic reference maps. Variable frames are introduced in order to group proteins into functional categories on three levels or into categories according to differential abundance during comparative proteome analyses. The software is easy to handle as it only requires uploading two digital files to a web site. An additional file including detailed information on all proteins can be combined with the primary map. Two different gel-based projects are presented to illustrate the capacity of GelMap for proteome annotation and evaluation.
KW - Proteome data annotation
KW - Public software
KW - The plant proteomic platform
KW - Two-dimensional gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=80052032987&partnerID=8YFLogxK
U2 - 10.15488/11659
DO - 10.15488/11659
M3 - Article
C2 - 21729775
AN - SCOPUS:80052032987
VL - 74
SP - 2214
EP - 2219
JO - Journal of proteomics
JF - Journal of proteomics
SN - 1874-3919
IS - 10
ER -