Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 413-5 |
| Seitenumfang | 3 |
| Fachzeitschrift | Pflugers Archiv European Journal of Physiology |
| Jahrgang | 422 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - Jan. 1993 |
Abstract
Using the double whole-cell recording technique we measured the gap junctional conductance (gj), while simultaneously monitoring the cytoplasmic free calcium concentration ([Ca2+i]) in isolated acinar cell pairs from the pancreas of mouse. Physiological concentrations of the secretagogue cholecystokinin-octapeptide (CCK-OP) elicited [Ca2+i] oscillations and after a delay in the minute range reduced gj. The results provide the first evidence suggesting that gj regulates the phase difference (delta phi) of [Ca2+i] oscillations between neighbouring cells not in an all or none process, but with decreasing absolute value of gj delta phi monotonically increases. CCK-OP induced electrical uncoupling as well as the corresponding increase of delta phi could be blocked completely by the protein kinase C inhibitor polymyxin B. The data support the hypothesis that Ca2+ flow through gap junctions synchronizes [Ca2+i] oscillations between jap junctional coupled cells.
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in: Pflugers Archiv European Journal of Physiology, Jahrgang 422, Nr. 4, 01.1993, S. 413-5.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Gap junctional conductance tunes phase difference of cholecystokinin evoked calcium oscillations in pairs of pancreatic acinar cells
AU - Ngezahayo, A
AU - Kolb, H A
PY - 1993/1
Y1 - 1993/1
N2 - Using the double whole-cell recording technique we measured the gap junctional conductance (gj), while simultaneously monitoring the cytoplasmic free calcium concentration ([Ca2+i]) in isolated acinar cell pairs from the pancreas of mouse. Physiological concentrations of the secretagogue cholecystokinin-octapeptide (CCK-OP) elicited [Ca2+i] oscillations and after a delay in the minute range reduced gj. The results provide the first evidence suggesting that gj regulates the phase difference (delta phi) of [Ca2+i] oscillations between neighbouring cells not in an all or none process, but with decreasing absolute value of gj delta phi monotonically increases. CCK-OP induced electrical uncoupling as well as the corresponding increase of delta phi could be blocked completely by the protein kinase C inhibitor polymyxin B. The data support the hypothesis that Ca2+ flow through gap junctions synchronizes [Ca2+i] oscillations between jap junctional coupled cells.
AB - Using the double whole-cell recording technique we measured the gap junctional conductance (gj), while simultaneously monitoring the cytoplasmic free calcium concentration ([Ca2+i]) in isolated acinar cell pairs from the pancreas of mouse. Physiological concentrations of the secretagogue cholecystokinin-octapeptide (CCK-OP) elicited [Ca2+i] oscillations and after a delay in the minute range reduced gj. The results provide the first evidence suggesting that gj regulates the phase difference (delta phi) of [Ca2+i] oscillations between neighbouring cells not in an all or none process, but with decreasing absolute value of gj delta phi monotonically increases. CCK-OP induced electrical uncoupling as well as the corresponding increase of delta phi could be blocked completely by the protein kinase C inhibitor polymyxin B. The data support the hypothesis that Ca2+ flow through gap junctions synchronizes [Ca2+i] oscillations between jap junctional coupled cells.
KW - Animals
KW - Calcium/metabolism
KW - Cholecystokinin/pharmacology
KW - Intercellular Junctions/drug effects
KW - Membrane Potentials/drug effects
KW - Mice
KW - Pancreas/cytology
U2 - 10.1007/BF00374302
DO - 10.1007/BF00374302
M3 - Article
C2 - 8437893
VL - 422
SP - 413
EP - 415
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 4
ER -