Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 395-401 |
| Seitenumfang | 7 |
| Fachzeitschrift | Biotechnology letters |
| Jahrgang | 31 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 4 Nov. 2008 |
Abstract
The lipase Lip2 of the edible basidiomycete, Pleurotus sapidus, is an extracellular enzyme capable of hydrolysing xanthophyll esters with high efficiency. The gene encoding Lip2 was expressed in Escherichia coli TOP10 using the gene III signal sequence to accumulate proteins in the periplasmatic space. The heterologous expression under control of the araBAD promoter led to the high level production of recombinant protein, mainly as inclusion bodies, but partially in a soluble and active form. A fusion with a C-terminal His tag was used for purification and immunochemical detection of the target protein. This is the first example of a heterologous expression and periplasmatic accumulation of a catalytically active lipase from a basidiomycete fungus.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Biotechnology letters, Jahrgang 31, Nr. 3, 04.11.2008, S. 395-401.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Functional expression of the lipase gene Lip2 of Pleurotus sapidus in Escherichia coli
AU - Zelena, Kateryna
AU - Krügener, Sven
AU - Lunkenbein, Stefan
AU - Zorn, Holger
AU - Berger, Ralf G.
N1 - Funding information: Acknowledgement Support of the work by the Deutsche Bundesstiftung Umwelt (AZ 13164) is gratefully acknowledged.
PY - 2008/11/4
Y1 - 2008/11/4
N2 - The lipase Lip2 of the edible basidiomycete, Pleurotus sapidus, is an extracellular enzyme capable of hydrolysing xanthophyll esters with high efficiency. The gene encoding Lip2 was expressed in Escherichia coli TOP10 using the gene III signal sequence to accumulate proteins in the periplasmatic space. The heterologous expression under control of the araBAD promoter led to the high level production of recombinant protein, mainly as inclusion bodies, but partially in a soluble and active form. A fusion with a C-terminal His tag was used for purification and immunochemical detection of the target protein. This is the first example of a heterologous expression and periplasmatic accumulation of a catalytically active lipase from a basidiomycete fungus.
AB - The lipase Lip2 of the edible basidiomycete, Pleurotus sapidus, is an extracellular enzyme capable of hydrolysing xanthophyll esters with high efficiency. The gene encoding Lip2 was expressed in Escherichia coli TOP10 using the gene III signal sequence to accumulate proteins in the periplasmatic space. The heterologous expression under control of the araBAD promoter led to the high level production of recombinant protein, mainly as inclusion bodies, but partially in a soluble and active form. A fusion with a C-terminal His tag was used for purification and immunochemical detection of the target protein. This is the first example of a heterologous expression and periplasmatic accumulation of a catalytically active lipase from a basidiomycete fungus.
KW - Basidiomycete
KW - Escherichia coli
KW - Heterologous expression
KW - Lipase
KW - Xanthophyll esters
UR - http://www.scopus.com/inward/record.url?scp=59149093793&partnerID=8YFLogxK
U2 - 10.1007/s10529-008-9870-7
DO - 10.1007/s10529-008-9870-7
M3 - Article
C2 - 18982247
AN - SCOPUS:59149093793
VL - 31
SP - 395
EP - 401
JO - Biotechnology letters
JF - Biotechnology letters
SN - 0141-5492
IS - 3
ER -