TY - JOUR

T1 - Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography

AU - Esatbeyoglu, T.

AU - Rodríguez-Werner, M.

AU - Winterhalter, P.

N1 - Funding information: We are grateful for the technical assistance of Janina Westphal and Josefine Ostberg. The authors thank the German Federal Ministry of Education and Research (BMBF—Bundesministerium für Bildung and Forschung) for financial support of the joint project “Dietary procyanidins” (Grant 0313828C). A. melanocarpa samples were kindly provided by Melanie Stürtz (Symrise, Holzminden, Germany) and Kelterei Walther (Dresden, Germany).

PY - 2017/7/1

Y1 - 2017/7/1

N2 - In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MS n and 1H- as well as 13C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.

AB - In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MS n and 1H- as well as 13C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.

KW - Anthocyanins

KW - Chokeberry

KW - Membrane adsorber

KW - Polyphenols

KW - Preparative countercurrent chromatography

UR - http://www.scopus.com/inward/record.url?scp=85007529349&partnerID=8YFLogxK

U2 - 10.1007/s00217-016-2837-3

DO - 10.1007/s00217-016-2837-3

M3 - Article

VL - 243

SP - 1261

EP - 1275

JO - European Food Research and Technology

JF - European Food Research and Technology

SN - 1438-2377

IS - 7

ER -