Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Multiphoton Microscopy in the Biomedical Sciences XV |
| Herausgeber (Verlag) | SPIE |
| ISBN (elektronisch) | 9781628414196 |
| Publikationsstatus | Veröffentlicht - 15 März 2015 |
| Veranstaltung | Multiphoton Microscopy in the Biomedical Sciences XV - San Francisco, USA / Vereinigte Staaten Dauer: 8 Feb. 2015 → 10 Feb. 2015 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Band | 9329 |
| ISSN (Print) | 1605-7422 |
Abstract
In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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Multiphoton Microscopy in the Biomedical Sciences XV. SPIE, 2015. 932912 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 9329).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Four-wave mixing microscopy
T2 - Multiphoton Microscopy in the Biomedical Sciences XV
AU - Ehmke, Tobias
AU - Knebl, Andreas
AU - Heisterkamp, Alexander
PY - 2015/3/15
Y1 - 2015/3/15
N2 - In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.
AB - In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.
KW - Four-wave mixing
KW - Nonlinear microscopy
KW - Nonlinear Optics
UR - http://www.scopus.com/inward/record.url?scp=84932109934&partnerID=8YFLogxK
U2 - 10.1117/12.2076743
DO - 10.1117/12.2076743
M3 - Conference contribution
AN - SCOPUS:84932109934
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences XV
PB - SPIE
Y2 - 8 February 2015 through 10 February 2015
ER -