Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 653-657 |
| Seitenumfang | 5 |
| Fachzeitschrift | Applied Microbiology and Biotechnology |
| Jahrgang | 42 |
| Ausgabenummer | 5 |
| Publikationsstatus | Veröffentlicht - Jan. 1995 |
| Extern publiziert | Ja |
Abstract
Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Applied Microbiology and Biotechnology, Jahrgang 42, Nr. 5, 01.1995, S. 653-657.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Fluorometric measurement of poly-β hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry
AU - Degelau, A.
AU - Scheper, T.
AU - Bailey, J. E.
AU - Guske, C.
PY - 1995/1
Y1 - 1995/1
N2 - Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.
AB - Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.
UR - http://www.scopus.com/inward/record.url?scp=0028984113&partnerID=8YFLogxK
U2 - 10.1007/BF00171939
DO - 10.1007/BF00171939
M3 - Article
AN - SCOPUS:0028984113
VL - 42
SP - 653
EP - 657
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -