Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 121-125 |
| Seitenumfang | 5 |
| Fachzeitschrift | Journal of Automated Methods and Management in Chemistry |
| Jahrgang | 21 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 1999 |
Abstract
A fully automated flow-injection immunoassay based on sandwich enzyme-linked immunosorbant assay (ELISA) is described for the model system: protein G-sepharose, rabbit IgG and horseradish peroxidase (HRP)-labelled protein A. After injecting rabbit IgG and HRP-labelled protein A into a cartridge containing protein G-sepharose sequentially, a mixture of hydrogen peroxide and the redox indicator, 2.2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) is passed through the cartridge. The HRP-labelled protein A bound in the cartridge is directly proportional to the concentration of rabbit IgG. The colour variation of ABTS caused during the reaction between HRP and H2O2 in the cartridge is defected photometrically. The whole assay procedure is controlled and evaluated by a computer. Rabbit IgG and HRP-labelled protein A are also detected by a fluorometer, which is introduced into the flow system. In the flow-injection sandwich ELISA, the slope of the calibration curve is 0.4491 in the range of 0 and 300 μg ml-1 rabbit IgG, while it is 0.1274 in the heterogeneous immunoassay. So the flow-injection sandwich ELISA system is found to be more sensitive than a heterogeneous immunoassay for the monitoring of the model protein.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Chemische Verfahrenstechnik (insg.)
- Chemische Verfahrenstechnik (sonstige)
- Physik und Astronomie (insg.)
- Instrumentierung
- Informatik (insg.)
- Angewandte Informatik
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in: Journal of Automated Methods and Management in Chemistry, Jahrgang 21, Nr. 4, 1999, S. 121-125.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Flow-injection sandwich ELISA for bioprocess monitoring
AU - Rhee, Jong I.I.
AU - Hagedorn, Jörg
AU - Scheper, Thomas
AU - Schügerl, Karl
PY - 1999
Y1 - 1999
N2 - A fully automated flow-injection immunoassay based on sandwich enzyme-linked immunosorbant assay (ELISA) is described for the model system: protein G-sepharose, rabbit IgG and horseradish peroxidase (HRP)-labelled protein A. After injecting rabbit IgG and HRP-labelled protein A into a cartridge containing protein G-sepharose sequentially, a mixture of hydrogen peroxide and the redox indicator, 2.2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) is passed through the cartridge. The HRP-labelled protein A bound in the cartridge is directly proportional to the concentration of rabbit IgG. The colour variation of ABTS caused during the reaction between HRP and H2O2 in the cartridge is defected photometrically. The whole assay procedure is controlled and evaluated by a computer. Rabbit IgG and HRP-labelled protein A are also detected by a fluorometer, which is introduced into the flow system. In the flow-injection sandwich ELISA, the slope of the calibration curve is 0.4491 in the range of 0 and 300 μg ml-1 rabbit IgG, while it is 0.1274 in the heterogeneous immunoassay. So the flow-injection sandwich ELISA system is found to be more sensitive than a heterogeneous immunoassay for the monitoring of the model protein.
AB - A fully automated flow-injection immunoassay based on sandwich enzyme-linked immunosorbant assay (ELISA) is described for the model system: protein G-sepharose, rabbit IgG and horseradish peroxidase (HRP)-labelled protein A. After injecting rabbit IgG and HRP-labelled protein A into a cartridge containing protein G-sepharose sequentially, a mixture of hydrogen peroxide and the redox indicator, 2.2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) is passed through the cartridge. The HRP-labelled protein A bound in the cartridge is directly proportional to the concentration of rabbit IgG. The colour variation of ABTS caused during the reaction between HRP and H2O2 in the cartridge is defected photometrically. The whole assay procedure is controlled and evaluated by a computer. Rabbit IgG and HRP-labelled protein A are also detected by a fluorometer, which is introduced into the flow system. In the flow-injection sandwich ELISA, the slope of the calibration curve is 0.4491 in the range of 0 and 300 μg ml-1 rabbit IgG, while it is 0.1274 in the heterogeneous immunoassay. So the flow-injection sandwich ELISA system is found to be more sensitive than a heterogeneous immunoassay for the monitoring of the model protein.
UR - http://www.scopus.com/inward/record.url?scp=0032816999&partnerID=8YFLogxK
U2 - 10.1155/S1463924699000140
DO - 10.1155/S1463924699000140
M3 - Article
AN - SCOPUS:0032816999
VL - 21
SP - 121
EP - 125
JO - Journal of Automated Methods and Management in Chemistry
JF - Journal of Automated Methods and Management in Chemistry
SN - 1463-9246
IS - 4
ER -