TY - JOUR

T1 - Flow rate dependent continuous hydrolysis of protein isolates

AU - Sewczyk, Tim

AU - Hoog Antink, Marieke

AU - Maas, Michael

AU - Kroll, Stephen

AU - Beutel, Sascha

N1 - Funding information: This research project was supported with a funding from the German Research Foundation (Deutsche Forschungsgemeinschaft) with the Grant Number 278836263. We would like to thank Novozymes A/S, Denmark for providing the Alcalase® 2.5 and the German Research Foundation for funding this project.

PY - 2018/2/10

Y1 - 2018/2/10

N2 - Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.

AB - Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.

KW - Bioactive peptides

KW - Continuous process

KW - Food protein

KW - Immobilization

KW - Proteases

UR - http://www.scopus.com/inward/record.url?scp=85041922676&partnerID=8YFLogxK

U2 - 10.1186/s13568-018-0548-9

DO - 10.1186/s13568-018-0548-9

M3 - Article

AN - SCOPUS:85041922676

VL - 8

JO - AMB EXPRESS

JF - AMB EXPRESS

SN - 2191-0855

M1 - 18

ER -