Flow cytometry of Escherichia coli for process monitoring

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OriginalspracheEnglisch
Seiten (von - bis)139-148
Seitenumfang10
FachzeitschriftJournal of biotechnology
Jahrgang5
Ausgabenummer2
PublikationsstatusVeröffentlicht - Apr. 1987

Abstract

A laser flow cytometer was used to study different Escherichia coli populations under various cultivation conditions. A host strain E. coli 5K was analyzed for cell size, protein and DNA-content during continuous cultivation. Also, a recombinant E. coli 5K(pHM12) strain (used for the intracellular production of penicillin-G acylase) was studied in regard to gene expression using different cytometric techniques. An argon ion laser (30 mW) and a 100 W high-pressure mercury lamp were used as light source in the cytometer. A new fluorogenic staining technique for intracellular penicillin-G acylase is described. Recombinant E. coli temperature sensitive cells were analyzed for intracellular fusion protein production due to temperature induction.

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Flow cytometry of Escherichia coli for process monitoring. / Scheper, T.; Hitzmann, B.; Rinas, U. et al.
in: Journal of biotechnology, Jahrgang 5, Nr. 2, 04.1987, S. 139-148.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Scheper T, Hitzmann B, Rinas U, Schügerl K. Flow cytometry of Escherichia coli for process monitoring. Journal of biotechnology. 1987 Apr;5(2):139-148. doi: 10.1016/0168-1656(87)90010-1
Scheper, T. ; Hitzmann, B. ; Rinas, U. et al. / Flow cytometry of Escherichia coli for process monitoring. in: Journal of biotechnology. 1987 ; Jahrgang 5, Nr. 2. S. 139-148.
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AU - Scheper, T.

AU - Hitzmann, B.

AU - Rinas, U.

AU - Schügerl, K.

PY - 1987/4

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KW - Intracellular enzyme activity

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