Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 388-394 |
| Seitenumfang | 7 |
| Fachzeitschrift | Engineering in life sciences |
| Jahrgang | 7 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 6 Juli 2007 |
Abstract
New membrane systems for the purification of proteins are presented. These disposable devices enable time saving screenings of downstream conditions with a small amount of protein solution. Furthermore, scaling up to the production level is directly possible since devices with effective membrane surfaces from 10 cm2 to 1 m2 are available. Several membranes like ion exchange membranes were investigated for the determination of protein binding capacities. Metal chelate membranes were used for ligand binding, which enables the determination of the membrane systems potential for affinity chromatography. Lysozyme and BSA are chosen for the determination of binding capacities of ion exchange membranes. Furthermore, Con A membranes, suitable for the isolation of glycoproteins, were developed and their binding properties were tested.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Umweltwissenschaften (insg.)
- Environmental engineering
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
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in: Engineering in life sciences, Jahrgang 7, Nr. 4, 06.07.2007, S. 388-394.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Fast Screening for the Purification of Proteins Using Membrane Adsorber Technology
AU - Harkensee, D.
AU - Kökpinar, Ö
AU - Walter, J.
AU - Kasper, C.
AU - Beutel, S.
AU - Reif, O. W.
AU - Scheper, T.
AU - Ulber, R.
PY - 2007/7/6
Y1 - 2007/7/6
N2 - New membrane systems for the purification of proteins are presented. These disposable devices enable time saving screenings of downstream conditions with a small amount of protein solution. Furthermore, scaling up to the production level is directly possible since devices with effective membrane surfaces from 10 cm2 to 1 m2 are available. Several membranes like ion exchange membranes were investigated for the determination of protein binding capacities. Metal chelate membranes were used for ligand binding, which enables the determination of the membrane systems potential for affinity chromatography. Lysozyme and BSA are chosen for the determination of binding capacities of ion exchange membranes. Furthermore, Con A membranes, suitable for the isolation of glycoproteins, were developed and their binding properties were tested.
AB - New membrane systems for the purification of proteins are presented. These disposable devices enable time saving screenings of downstream conditions with a small amount of protein solution. Furthermore, scaling up to the production level is directly possible since devices with effective membrane surfaces from 10 cm2 to 1 m2 are available. Several membranes like ion exchange membranes were investigated for the determination of protein binding capacities. Metal chelate membranes were used for ligand binding, which enables the determination of the membrane systems potential for affinity chromatography. Lysozyme and BSA are chosen for the determination of binding capacities of ion exchange membranes. Furthermore, Con A membranes, suitable for the isolation of glycoproteins, were developed and their binding properties were tested.
KW - Downstream processing
KW - Membranes
KW - Proteins
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=34547403135&partnerID=8YFLogxK
U2 - 10.1002/elsc.200720194
DO - 10.1002/elsc.200720194
M3 - Article
AN - SCOPUS:34547403135
VL - 7
SP - 388
EP - 394
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 4
ER -