Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 145-153 |
| Seitenumfang | 9 |
| Fachzeitschrift | Journal of biotechnology |
| Jahrgang | 59 |
| Ausgabenummer | 1-2 |
| Publikationsstatus | Veröffentlicht - Dez. 1997 |
Abstract
An automated immunoassay, with one affinity component immobilized on a solid surface, has been developed to monitor the production of different immunoglobulins during mammalian cell cultivation processes. The whole analysis device is based on the principle of flow injection analysis (FIA) and a cartridge with the immobilized affinity component is implemented into the FIA system. This cartridge is filled with a carrier material to which protein G is covalently bound. After sample injection, binding of the IgG on the protein G within the cartridge takes place while after a washing step, the IgGs are eluted by a pH shift, and the IgG concentration is monitored via fluorescence. In the automated immunoassay, undiluted cell free samples from the reactor or from down-stream processing can be analyzed directly. Due to the separation the IgG can be detected without interference from other sample components by protein fluorescence. The results are obtained with analysis times below 6 min. Sample volumes of less than 100 μl may be used. The assay is sensitive to concentrations from 5 up to 500 μg ml-1. Using this FIA-System, immunoglobulins G, produced in different media, were successfully monitored. The results of the assay were validated by ELISA.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Journal of biotechnology, Jahrgang 59, Nr. 1-2, 12.1997, S. 145-153.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Fast on-line flow injection analysis system for IgG monitoring in bioprocesses
AU - Reinecke, Martin
AU - Scheper, Thomas
PY - 1997/12
Y1 - 1997/12
N2 - An automated immunoassay, with one affinity component immobilized on a solid surface, has been developed to monitor the production of different immunoglobulins during mammalian cell cultivation processes. The whole analysis device is based on the principle of flow injection analysis (FIA) and a cartridge with the immobilized affinity component is implemented into the FIA system. This cartridge is filled with a carrier material to which protein G is covalently bound. After sample injection, binding of the IgG on the protein G within the cartridge takes place while after a washing step, the IgGs are eluted by a pH shift, and the IgG concentration is monitored via fluorescence. In the automated immunoassay, undiluted cell free samples from the reactor or from down-stream processing can be analyzed directly. Due to the separation the IgG can be detected without interference from other sample components by protein fluorescence. The results are obtained with analysis times below 6 min. Sample volumes of less than 100 μl may be used. The assay is sensitive to concentrations from 5 up to 500 μg ml-1. Using this FIA-System, immunoglobulins G, produced in different media, were successfully monitored. The results of the assay were validated by ELISA.
AB - An automated immunoassay, with one affinity component immobilized on a solid surface, has been developed to monitor the production of different immunoglobulins during mammalian cell cultivation processes. The whole analysis device is based on the principle of flow injection analysis (FIA) and a cartridge with the immobilized affinity component is implemented into the FIA system. This cartridge is filled with a carrier material to which protein G is covalently bound. After sample injection, binding of the IgG on the protein G within the cartridge takes place while after a washing step, the IgGs are eluted by a pH shift, and the IgG concentration is monitored via fluorescence. In the automated immunoassay, undiluted cell free samples from the reactor or from down-stream processing can be analyzed directly. Due to the separation the IgG can be detected without interference from other sample components by protein fluorescence. The results are obtained with analysis times below 6 min. Sample volumes of less than 100 μl may be used. The assay is sensitive to concentrations from 5 up to 500 μg ml-1. Using this FIA-System, immunoglobulins G, produced in different media, were successfully monitored. The results of the assay were validated by ELISA.
KW - Binding assay
KW - Bioprocess monitoring
KW - Flow injection analysis
KW - IgG
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=0031576556&partnerID=8YFLogxK
U2 - 10.1016/S0168-1656(97)00177-6
DO - 10.1016/S0168-1656(97)00177-6
M3 - Article
C2 - 9487722
AN - SCOPUS:0031576556
VL - 59
SP - 145
EP - 153
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 1-2
ER -