Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 100008 |
| Fachzeitschrift | Engineering Microbiology |
| Jahrgang | 2 |
| Ausgabenummer | 1 |
| Frühes Online-Datum | 26 Nov. 2021 |
| Publikationsstatus | Veröffentlicht - März 2022 |
| Extern publiziert | Ja |
Abstract
Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of research. Previously, it has been shown that various heterologous redox enzymes could be expressed in these microorganisms. Here we demonstrated the successful inducer-free expression of α-keto-acid dehydrogenases (L-HicDH and D-HicDH) from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp. PCC 6803 ΔhoxYH mutant using replicative plasmids. While the L-HicDH showed poor activity limited by the amount of expressed enzyme, the D-HicDH was applied both in vivo and in vitro, transforming the selected α-keto acids to the corresponding optically pure (R)-α-hydroxy acids (ee >99%) in up to 53% and 90% conversion, respectively.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie, Genetik und Molekularbiologie (sonstige)
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
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in: Engineering Microbiology, Jahrgang 2, Nr. 1, 100008, 03.2022.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 ΔhoxYH
AU - Jurkaš, Valentina
AU - Winkler, Christoph K.
AU - Poschenrieder, Silvan
AU - Oliveira, Paulo
AU - Pacheco, Catarina C.
AU - Ferreira, Eunice A.
AU - Weissensteiner, Florian
AU - Santis, Piera De
AU - Kara, Selin
AU - Kourist, Robert
AU - Tamagnini, Paula
AU - Kroutil, Wolfgang
N1 - Publisher Copyright: © 2021
PY - 2022/3
Y1 - 2022/3
N2 - Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of research. Previously, it has been shown that various heterologous redox enzymes could be expressed in these microorganisms. Here we demonstrated the successful inducer-free expression of α-keto-acid dehydrogenases (L-HicDH and D-HicDH) from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp. PCC 6803 ΔhoxYH mutant using replicative plasmids. While the L-HicDH showed poor activity limited by the amount of expressed enzyme, the D-HicDH was applied both in vivo and in vitro, transforming the selected α-keto acids to the corresponding optically pure (R)-α-hydroxy acids (ee >99%) in up to 53% and 90% conversion, respectively.
AB - Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of research. Previously, it has been shown that various heterologous redox enzymes could be expressed in these microorganisms. Here we demonstrated the successful inducer-free expression of α-keto-acid dehydrogenases (L-HicDH and D-HicDH) from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp. PCC 6803 ΔhoxYH mutant using replicative plasmids. While the L-HicDH showed poor activity limited by the amount of expressed enzyme, the D-HicDH was applied both in vivo and in vitro, transforming the selected α-keto acids to the corresponding optically pure (R)-α-hydroxy acids (ee >99%) in up to 53% and 90% conversion, respectively.
KW - Biocatalysis
KW - Cyanobacteria
KW - Hydroxyisocaproate dehydrogenase
KW - Ketoacid dehydrogenase
KW - Photobiotransformation
KW - Synechocystis sp. PCC 6803
UR - http://www.scopus.com/inward/record.url?scp=85137770966&partnerID=8YFLogxK
U2 - 10.1016/j.engmic.2021.100008
DO - 10.1016/j.engmic.2021.100008
M3 - Article
VL - 2
JO - Engineering Microbiology
JF - Engineering Microbiology
IS - 1
M1 - 100008
ER -