TY - JOUR

T1 - Exploring the possibility of cryopreservation of feline and canine erythrocytes by rapid freezing with penetrating and non-penetrating cryoprotectants

AU - Pogozhykh, Denys

AU - Pakhomova, Yuliya

AU - Pervushina, Olga

AU - Hofmann, Nicola

AU - Glasmacher, Birgit

AU - Zhegunov, Gennadiy

N1 - Funding Information: The research conduction and article preparation were funded by the cooperating institutes: 1. Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany; 2. Institute for Multiphase Processes, Leibniz Universitaet Hannover, Hannover, Germany; 3. Kharkiv State Zooveterinary Academy, Mala Danylivka, Kharkiv Region, Ukraine; 4. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine. Besides, the research was partially supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, EXC 62/1) through travel funding by the Cluster of Excellence REBIRTH. Decisions in study design; in the collection, analysis and interpretation of data; in the writing of the report; and the decision to submit the article for publication were taken by the senior authors. Publisher Copyright: © 2017 Pogozhykh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2017/1/10

Y1 - 2017/1/10

N2 - Efficient application of veterinary blood transfusion approaches for small companion animals requires readily available supply of the donor material. This can be achieved by developing of effective biobanking technologies allowing long-term storage of donor blood components via cryopreservation. Transfusion of an erythrocyte concentrate allows the successful correction of various hematological pathologies, severe bleeding, and etc. While in the past there were several approaches to cryopreserve red blood cells of dogs, to our knowledge there is virtually no data on cryopreservation of feline erythrocytes. In this paper, we performed a comprehensive parameter optimization for low temperature storage of RBCs of both species. Here, the efficiency of single-component and multicomponent cryoprotective media as well as necessary time of pre-incubation with penetrating and non-penetrating cryoprotectants prior to rapid freezing is analyzed. This study showed that glycerol was not sufficient for cryopreservation of red blood cells of the studied species under the investigated conditions. Application of 10% (v/v) ME2SO allowed for a significant reduction of canine and feline erythrocytes hemolysis after thawing. 17.5% hydroxyethyl starch demonstrated the highest cryoprotective activity for both species. It was found that dog RBCs should be incubated in cryoprotective media for 30 min at 22°C prior to freezing, while for cat RBCs 20 min is sufficient. Combination of CPAs was less effective. Presented data may be considered in further studies in veterinary transfusion and blood banking optimization.

AB - Efficient application of veterinary blood transfusion approaches for small companion animals requires readily available supply of the donor material. This can be achieved by developing of effective biobanking technologies allowing long-term storage of donor blood components via cryopreservation. Transfusion of an erythrocyte concentrate allows the successful correction of various hematological pathologies, severe bleeding, and etc. While in the past there were several approaches to cryopreserve red blood cells of dogs, to our knowledge there is virtually no data on cryopreservation of feline erythrocytes. In this paper, we performed a comprehensive parameter optimization for low temperature storage of RBCs of both species. Here, the efficiency of single-component and multicomponent cryoprotective media as well as necessary time of pre-incubation with penetrating and non-penetrating cryoprotectants prior to rapid freezing is analyzed. This study showed that glycerol was not sufficient for cryopreservation of red blood cells of the studied species under the investigated conditions. Application of 10% (v/v) ME2SO allowed for a significant reduction of canine and feline erythrocytes hemolysis after thawing. 17.5% hydroxyethyl starch demonstrated the highest cryoprotective activity for both species. It was found that dog RBCs should be incubated in cryoprotective media for 30 min at 22°C prior to freezing, while for cat RBCs 20 min is sufficient. Combination of CPAs was less effective. Presented data may be considered in further studies in veterinary transfusion and blood banking optimization.

UR - http://www.scopus.com/inward/record.url?scp=85009106679&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0169689

DO - 10.1371/journal.pone.0169689

M3 - Article

C2 - 28072844

AN - SCOPUS:85009106679

VL - 12

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 1

M1 - e0169689

ER -