Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Quantitative Phase Imaging |
| Herausgeber/-innen | YongKeun Park, Gabriel Popescu |
| Herausgeber (Verlag) | SPIE |
| ISBN (elektronisch) | 9781628414264 |
| Publikationsstatus | Veröffentlicht - 11 März 2015 |
| Extern publiziert | Ja |
| Veranstaltung | 1st Conference on Quantitative Phase Imaging, QPI 2015 - San Francisco, USA / Vereinigte Staaten Dauer: 7 Feb. 2015 → 10 Feb. 2015 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Band | 9336 |
| ISSN (Print) | 1605-7422 |
Abstract
Our research combines Digital Holographic Microscopy (DHM) and fluorescence microscopy to study the basic mechanisms of gold nanoparticle mediated laser manipulation. Herein we describe the technical aspects of the setup and holographic image reconstruction. Furthermore, results pertaining to cell volume change and calcium response of cells in laser manipulation will be presented and discussed. For the reconstruction of phase images from fringe image data, a phase unwrapping algorithm is presented that shows great potential to cope with the vast amount of data that was captured. This algorithm is a hybrid between a tile unwrapping technique and a path following unwrapper. It combines the robustness of a path following algorithm and a parallelizable tile unwrapping preprocessing step. The experimental setup enables simultaneous acquisition of fluorescence and phase images. For cell manipulation, a picosecond laser was coupled into the setup and weakly focused on cells incubated with gold nanoparticles. To study the cell volume change in the first minute, phase images were captured with a frame rate of 33 fps. Fluorescence images yielded the calcium signal of the cells as well as the dynamics of the F-actin cytoskeleton after irradiation. The setup is suitable to study fast changes in biophysical and morphological parameters of cells by recording phase and -uorescence images for different laser based cell manipulation scenarios.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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- BibTex
- RIS
Quantitative Phase Imaging. Hrsg. / YongKeun Park; Gabriel Popescu. SPIE, 2015. 93360G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 9336).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Experimental setup combining digital holographic microscopy (DHM) and fluorescence imaging to study gold nanoparticle mediated laser manipulation
AU - Antonopoulos, Georgios C.
AU - Rakoski, Mirko S.
AU - Steltner, Benjamin
AU - Kalies, Stefan
AU - Ripken, Tammo
AU - Meyer, Heiko
N1 - Publisher Copyright: © 2015 SPIE.
PY - 2015/3/11
Y1 - 2015/3/11
N2 - Our research combines Digital Holographic Microscopy (DHM) and fluorescence microscopy to study the basic mechanisms of gold nanoparticle mediated laser manipulation. Herein we describe the technical aspects of the setup and holographic image reconstruction. Furthermore, results pertaining to cell volume change and calcium response of cells in laser manipulation will be presented and discussed. For the reconstruction of phase images from fringe image data, a phase unwrapping algorithm is presented that shows great potential to cope with the vast amount of data that was captured. This algorithm is a hybrid between a tile unwrapping technique and a path following unwrapper. It combines the robustness of a path following algorithm and a parallelizable tile unwrapping preprocessing step. The experimental setup enables simultaneous acquisition of fluorescence and phase images. For cell manipulation, a picosecond laser was coupled into the setup and weakly focused on cells incubated with gold nanoparticles. To study the cell volume change in the first minute, phase images were captured with a frame rate of 33 fps. Fluorescence images yielded the calcium signal of the cells as well as the dynamics of the F-actin cytoskeleton after irradiation. The setup is suitable to study fast changes in biophysical and morphological parameters of cells by recording phase and -uorescence images for different laser based cell manipulation scenarios.
AB - Our research combines Digital Holographic Microscopy (DHM) and fluorescence microscopy to study the basic mechanisms of gold nanoparticle mediated laser manipulation. Herein we describe the technical aspects of the setup and holographic image reconstruction. Furthermore, results pertaining to cell volume change and calcium response of cells in laser manipulation will be presented and discussed. For the reconstruction of phase images from fringe image data, a phase unwrapping algorithm is presented that shows great potential to cope with the vast amount of data that was captured. This algorithm is a hybrid between a tile unwrapping technique and a path following unwrapper. It combines the robustness of a path following algorithm and a parallelizable tile unwrapping preprocessing step. The experimental setup enables simultaneous acquisition of fluorescence and phase images. For cell manipulation, a picosecond laser was coupled into the setup and weakly focused on cells incubated with gold nanoparticles. To study the cell volume change in the first minute, phase images were captured with a frame rate of 33 fps. Fluorescence images yielded the calcium signal of the cells as well as the dynamics of the F-actin cytoskeleton after irradiation. The setup is suitable to study fast changes in biophysical and morphological parameters of cells by recording phase and -uorescence images for different laser based cell manipulation scenarios.
KW - digital holography
KW - fluorescence microscopy
KW - gold nanoparticle mediated laser manipulation
KW - laser transfection
KW - phase unwrapping
UR - http://www.scopus.com/inward/record.url?scp=84931413075&partnerID=8YFLogxK
U2 - 10.1117/12.2079322
DO - 10.1117/12.2079322
M3 - Conference contribution
AN - SCOPUS:84931413075
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Quantitative Phase Imaging
A2 - Park, YongKeun
A2 - Popescu, Gabriel
PB - SPIE
T2 - 1st Conference on Quantitative Phase Imaging, QPI 2015
Y2 - 7 February 2015 through 10 February 2015
ER -