Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 5421-31 |
| Seitenumfang | 11 |
| Fachzeitschrift | Anticancer research |
| Jahrgang | 33 |
| Ausgabenummer | 12 |
| Publikationsstatus | Veröffentlicht - Dez. 2013 |
Abstract
BACKGROUND/AIM: In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.
MATERIALS AND METHODS: Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.
RESULTS: Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.
CONCLUSION: The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
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in: Anticancer research, Jahrgang 33, Nr. 12, 12.2013, S. 5421-31.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Evaluation of stem cell marker gene expression in canine prostate carcinoma- and prostate cyst-derived cell lines
AU - Moulay, Mohammed
AU - Liu, Wen
AU - Willenbrock, Saskia
AU - Sterenczak, Katharina Anna
AU - Carlson, Regina
AU - Ngezahayo, Anaclet
AU - Murua Escobar, Hugo
AU - Nolte, Ingo
PY - 2013/12
Y1 - 2013/12
N2 - BACKGROUND/AIM: In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.MATERIALS AND METHODS: Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.RESULTS: Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.CONCLUSION: The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
AB - BACKGROUND/AIM: In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.MATERIALS AND METHODS: Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.RESULTS: Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.CONCLUSION: The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
KW - Animals
KW - Base Sequence
KW - Biomarkers/metabolism
KW - Cell Line
KW - Cysts/genetics
KW - DNA Primers
KW - Dogs
KW - Gene Expression
KW - Male
KW - Prostatic Diseases/genetics
KW - Prostatic Neoplasms/genetics
KW - Real-Time Polymerase Chain Reaction
KW - Stem Cells/metabolism
U2 - doi: 10.1016/j.yexcr.2013.03.016
DO - doi: 10.1016/j.yexcr.2013.03.016
M3 - Article
C2 - 24324078
VL - 33
SP - 5421
EP - 5431
JO - Anticancer research
JF - Anticancer research
SN - 0250-7005
IS - 12
ER -