Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandAufsatz in KonferenzbandForschungPeer-Review

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Externe Organisationen

  • Université de Strasbourg
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Details

OriginalspracheEnglisch
Titel des SammelwerksActa Horticulturae
Herausgeber (Verlag)International Society for Horticultural Science
Seiten239-242
Seitenumfang4
ISBN (Print)9789066051102
PublikationsstatusVeröffentlicht - 28 Feb. 2010

Publikationsreihe

NameActa Horticulturae
Band855
ISSN (Print)0567-7572

Abstract

Comprehensive proteomic characterizations were performed aiming to create proteome reference maps for somatic and zygotic embryos of Cyclamen persicum. Separation by two dimensional isoelectric focusing - sodium dodecyl sulfate polyacrylamide gel electrophoresis led to a resolution of more than 800 protein spots for each tissue. Approximately 70% of the spots likewise appeared in both zygotic and in somatic embryo's protein fractions. However, differential gel electrophoresis analyses revealed pronounced differences in abundances for the majority of proteins present in both tissues. MS analyses for 300 reproducible spots in total (263 of the zygotic embryos' protein fraction and 37 spots appearing specifically in the somatic embryos' proteome) led to identification of 261 proteins, 35 of which were specific or highly abundant in gels of the somatic embryo's tissue. Most identified proteins were found to be involved in glycolysis or gluconeogenesis and stress response pathways.

ASJC Scopus Sachgebiete

  • Agrar- und Biowissenschaften (insg.)
  • Gartenbau

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Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill. / Rode, C.; Braun, H. P.; Gallien, S. et al.
Acta Horticulturae. International Society for Horticultural Science, 2010. S. 239-242 (Acta Horticulturae; Band 855).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandAufsatz in KonferenzbandForschungPeer-Review

Rode, C, Braun, HP, Gallien, S, Heintz, D, Van Dorsselaer, A & Winkelmann, T 2010, Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill. in Acta Horticulturae. Acta Horticulturae, Bd. 855, International Society for Horticultural Science, S. 239-242. https://doi.org/10.15488/11661, https://doi.org/10.17660/ActaHortic.2010.855.35
Rode, C., Braun, H. P., Gallien, S., Heintz, D., Van Dorsselaer, A., & Winkelmann, T. (2010). Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill. In Acta Horticulturae (S. 239-242). (Acta Horticulturae; Band 855). International Society for Horticultural Science. https://doi.org/10.15488/11661, https://doi.org/10.17660/ActaHortic.2010.855.35
Rode C, Braun HP, Gallien S, Heintz D, Van Dorsselaer A, Winkelmann T. Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill. in Acta Horticulturae. International Society for Horticultural Science. 2010. S. 239-242. (Acta Horticulturae). doi: 10.15488/11661, 10.17660/ActaHortic.2010.855.35
Rode, C. ; Braun, H. P. ; Gallien, S. et al. / Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill. Acta Horticulturae. International Society for Horticultural Science, 2010. S. 239-242 (Acta Horticulturae).
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abstract = "Comprehensive proteomic characterizations were performed aiming to create proteome reference maps for somatic and zygotic embryos of Cyclamen persicum. Separation by two dimensional isoelectric focusing - sodium dodecyl sulfate polyacrylamide gel electrophoresis led to a resolution of more than 800 protein spots for each tissue. Approximately 70% of the spots likewise appeared in both zygotic and in somatic embryo's protein fractions. However, differential gel electrophoresis analyses revealed pronounced differences in abundances for the majority of proteins present in both tissues. MS analyses for 300 reproducible spots in total (263 of the zygotic embryos' protein fraction and 37 spots appearing specifically in the somatic embryos' proteome) led to identification of 261 proteins, 35 of which were specific or highly abundant in gels of the somatic embryo's tissue. Most identified proteins were found to be involved in glycolysis or gluconeogenesis and stress response pathways.",
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