TY - JOUR

T1 - Establishing embryogenic suspension cultures in Cyclamen persicum 'Purple Flamed'

AU - Winkelmann, T.

AU - Hohe, A.

AU - Schwenkel, H. G.

PY - 1998

Y1 - 1998

N2 - As compared to callus, suspension cultures offer the advantages of reduced labour for maintaining cultures as well as faster growth in case of somatic embryogenesis in Cyclamen persicum 'Purple Flamed'. When transferred to bioreactors growth and differentiation can be controlled and automatization becomes possible. Both, a non-embryogenic and an embryogenic callus line derived from somatic tissue of unpollinated ovules of the same plant, were used as inocula for long-term suspension cultures showing stable growth for more than one year. Critical factors for the suspension establishment were the starting density, the growth phase of the embryogenic callus and the dilution and subculture scheme. Growth curves for embryogenic and non-embryogenic suspensions are presented. Both types of suspensions were successfully established in bioreactors in first trials. The regeneration of globular-shaped somatic embryos in high frequencies was observed after plating the fraction 200-500 μm on hormone-free medium. However, the conversion to normal plantlets has to be optimised, since pronounced root growth was observed in somatic embryos derived from callus and suspension cultures. At the moment a yield of about 90.000 plantlets from 1 I suspension can be achieved representing only a small portion of the embryogenic potential of the cultures.

AB - As compared to callus, suspension cultures offer the advantages of reduced labour for maintaining cultures as well as faster growth in case of somatic embryogenesis in Cyclamen persicum 'Purple Flamed'. When transferred to bioreactors growth and differentiation can be controlled and automatization becomes possible. Both, a non-embryogenic and an embryogenic callus line derived from somatic tissue of unpollinated ovules of the same plant, were used as inocula for long-term suspension cultures showing stable growth for more than one year. Critical factors for the suspension establishment were the starting density, the growth phase of the embryogenic callus and the dilution and subculture scheme. Growth curves for embryogenic and non-embryogenic suspensions are presented. Both types of suspensions were successfully established in bioreactors in first trials. The regeneration of globular-shaped somatic embryos in high frequencies was observed after plating the fraction 200-500 μm on hormone-free medium. However, the conversion to normal plantlets has to be optimised, since pronounced root growth was observed in somatic embryos derived from callus and suspension cultures. At the moment a yield of about 90.000 plantlets from 1 I suspension can be achieved representing only a small portion of the embryogenic potential of the cultures.

KW - 2,4-D

KW - 2iP

KW - Bioreactor culture

KW - Cyclamen persicum

KW - Somatic embryogenesis

KW - Suspension culture

UR - http://www.scopus.com/inward/record.url?scp=0031970524&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031970524

VL - 12

SP - 25

EP - 30

JO - Advances in Horticultural Science

JF - Advances in Horticultural Science

SN - 0394-6169

IS - 1

ER -