Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 186-94 |
| Seitenumfang | 9 |
| Fachzeitschrift | Trends in plant science |
| Jahrgang | 18 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - Apr. 2013 |
Abstract
The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.
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in: Trends in plant science, Jahrgang 18, Nr. 4, 04.2013, S. 186-94.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Essential nucleoid proteins in early chloroplast development
AU - Pfalz, Jeannette
AU - Pfannschmidt, Thomas
N1 - Funding information: Our work is supported by DFG grants PF667-4 (J.P.) and PF323-4 and PF323-5 (T.P.). We thank Sebastian Steiner and Severin Sasso for their critical discussion.
PY - 2013/4
Y1 - 2013/4
N2 - The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.
AB - The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.
KW - Chloroplast Proteins/genetics
KW - Chloroplasts/genetics
KW - DNA, Chloroplast/genetics
KW - DNA-Directed RNA Polymerases/genetics
KW - Gene Expression Regulation, Plant
KW - Genome, Chloroplast/genetics
KW - Models, Biological
KW - Phenotype
KW - Transcription, Genetic
U2 - 10.1016/j.tplants.2012.11.003
DO - 10.1016/j.tplants.2012.11.003
M3 - Article
C2 - 23246438
VL - 18
SP - 186
EP - 194
JO - Trends in plant science
JF - Trends in plant science
SN - 1360-1385
IS - 4
ER -