Essential nucleoid proteins in early chloroplast development

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OriginalspracheEnglisch
Seiten (von - bis)186-94
Seitenumfang9
FachzeitschriftTrends in plant science
Jahrgang18
Ausgabenummer4
PublikationsstatusVeröffentlicht - Apr. 2013

Abstract

The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.

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Essential nucleoid proteins in early chloroplast development. / Pfalz, Jeannette; Pfannschmidt, Thomas.
in: Trends in plant science, Jahrgang 18, Nr. 4, 04.2013, S. 186-94.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Pfalz J, Pfannschmidt T. Essential nucleoid proteins in early chloroplast development. Trends in plant science. 2013 Apr;18(4):186-94. doi: 10.1016/j.tplants.2012.11.003
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abstract = "The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.",
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AU - Pfalz, Jeannette

AU - Pfannschmidt, Thomas

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AB - The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.

KW - Chloroplast Proteins/genetics

KW - Chloroplasts/genetics

KW - DNA, Chloroplast/genetics

KW - DNA-Directed RNA Polymerases/genetics

KW - Gene Expression Regulation, Plant

KW - Genome, Chloroplast/genetics

KW - Models, Biological

KW - Phenotype

KW - Transcription, Genetic

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JO - Trends in plant science

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SN - 1360-1385

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