ENZYMATIC PRODUCTION OF L-AMINO ACID WITH CONTINUOUS COENZYME REGENERATION BY LIQUID MEMBRANE TECHNIQUE.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Organisationseinheiten

Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)345-350
Seitenumfang6
FachzeitschriftGerman Chemical Engineering
Jahrgang8
Ausgabenummer6
PublikationsstatusVeröffentlicht - Nov. 1985

Abstract

L-Leucine-dehydrogenase (LEUDH) catalyzes the reductive amination of alpha -ketoisocaproate to L-leucine. During this reaction, NADH is oxidized and simultaneously reduced by formatedehydrogenase (FDH). The two enzymes and the coenzyme were immobilized in a liquid membrane emulsion. Several organic phases and emulsifiers were tested, in order to obtain suitable immobilization systems. Batch tests were carried out for optimization of operating conditions and determination of kinetic parameters. Mass transfer through the membrane is the rate determining step. Permeation coefficients for the substrates and product were determined on solid-supported liquid membranes.

ASJC Scopus Sachgebiete

Zitieren

ENZYMATIC PRODUCTION OF L-AMINO ACID WITH CONTINUOUS COENZYME REGENERATION BY LIQUID MEMBRANE TECHNIQUE. / Makryaleas, Kyriakos; Scheper, Thomas; Schuegerl, Karl et al.
in: German Chemical Engineering, Jahrgang 8, Nr. 6, 11.1985, S. 345-350.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Download
@article{af04cd9cb67743d185d4c5de63533676,
title = "ENZYMATIC PRODUCTION OF L-AMINO ACID WITH CONTINUOUS COENZYME REGENERATION BY LIQUID MEMBRANE TECHNIQUE.",
abstract = "L-Leucine-dehydrogenase (LEUDH) catalyzes the reductive amination of alpha -ketoisocaproate to L-leucine. During this reaction, NADH is oxidized and simultaneously reduced by formatedehydrogenase (FDH). The two enzymes and the coenzyme were immobilized in a liquid membrane emulsion. Several organic phases and emulsifiers were tested, in order to obtain suitable immobilization systems. Batch tests were carried out for optimization of operating conditions and determination of kinetic parameters. Mass transfer through the membrane is the rate determining step. Permeation coefficients for the substrates and product were determined on solid-supported liquid membranes.",
author = "Kyriakos Makryaleas and Thomas Scheper and Karl Schuegerl and Kula, {Maria Regina}",
year = "1985",
month = nov,
language = "English",
volume = "8",
pages = "345--350",
journal = "German Chemical Engineering",
issn = "0343-5539",
publisher = "Wiley-VCH Verlag",
number = "6",

}

Download

TY - JOUR

T1 - ENZYMATIC PRODUCTION OF L-AMINO ACID WITH CONTINUOUS COENZYME REGENERATION BY LIQUID MEMBRANE TECHNIQUE.

AU - Makryaleas, Kyriakos

AU - Scheper, Thomas

AU - Schuegerl, Karl

AU - Kula, Maria Regina

PY - 1985/11

Y1 - 1985/11

N2 - L-Leucine-dehydrogenase (LEUDH) catalyzes the reductive amination of alpha -ketoisocaproate to L-leucine. During this reaction, NADH is oxidized and simultaneously reduced by formatedehydrogenase (FDH). The two enzymes and the coenzyme were immobilized in a liquid membrane emulsion. Several organic phases and emulsifiers were tested, in order to obtain suitable immobilization systems. Batch tests were carried out for optimization of operating conditions and determination of kinetic parameters. Mass transfer through the membrane is the rate determining step. Permeation coefficients for the substrates and product were determined on solid-supported liquid membranes.

AB - L-Leucine-dehydrogenase (LEUDH) catalyzes the reductive amination of alpha -ketoisocaproate to L-leucine. During this reaction, NADH is oxidized and simultaneously reduced by formatedehydrogenase (FDH). The two enzymes and the coenzyme were immobilized in a liquid membrane emulsion. Several organic phases and emulsifiers were tested, in order to obtain suitable immobilization systems. Batch tests were carried out for optimization of operating conditions and determination of kinetic parameters. Mass transfer through the membrane is the rate determining step. Permeation coefficients for the substrates and product were determined on solid-supported liquid membranes.

UR - http://www.scopus.com/inward/record.url?scp=0022151621&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0022151621

VL - 8

SP - 345

EP - 350

JO - German Chemical Engineering

JF - German Chemical Engineering

SN - 0343-5539

IS - 6

ER -

Von denselben Autoren