Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 623-628 |
| Seitenumfang | 6 |
| Fachzeitschrift | Enzyme and microbial technology |
| Jahrgang | 32 |
| Ausgabenummer | 5 |
| Publikationsstatus | Veröffentlicht - 11 Feb. 2003 |
Abstract
The carotenoids lutein and capsanthin, widely used as colorants, mainly occur as esters of fatty acids in their producer plants marigold and paprika. The enzymatic hydrolysis of these esters is reported here. Out of 26 commercial lipases, fastest ester hydrolysis under conditions was obtained using an enzyme from Candida antarctica (69% release of capsanthin, 44% of lutein from the respective oleoresins). Bile salts were essential for the activity of all commercial enzymes. A novel hydrolase was discovered in the mycelium-free supernatant of submerged cultures of the edible basidiomycete Pleurotus sapidus. The fungal enzyme cleaved several carotenoid esters almost completely and in the absence of bile salts.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Enzyme and microbial technology, Jahrgang 32, Nr. 5, 11.02.2003, S. 623-628.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Enzymatic hydrolysis of carotenoid esters of marigold flowers (Tagetes erecta L.) and red paprika (Capsicum annuum L.) by commercial lipases and Pleurotus sapidus extracellular lipase
AU - Zorn, Holger
AU - Breithaupt, Dietmar E.
AU - Takenberg, Meike
AU - Schwack, Wolfgang
AU - Berger, Ralf G.
PY - 2003/2/11
Y1 - 2003/2/11
N2 - The carotenoids lutein and capsanthin, widely used as colorants, mainly occur as esters of fatty acids in their producer plants marigold and paprika. The enzymatic hydrolysis of these esters is reported here. Out of 26 commercial lipases, fastest ester hydrolysis under conditions was obtained using an enzyme from Candida antarctica (69% release of capsanthin, 44% of lutein from the respective oleoresins). Bile salts were essential for the activity of all commercial enzymes. A novel hydrolase was discovered in the mycelium-free supernatant of submerged cultures of the edible basidiomycete Pleurotus sapidus. The fungal enzyme cleaved several carotenoid esters almost completely and in the absence of bile salts.
AB - The carotenoids lutein and capsanthin, widely used as colorants, mainly occur as esters of fatty acids in their producer plants marigold and paprika. The enzymatic hydrolysis of these esters is reported here. Out of 26 commercial lipases, fastest ester hydrolysis under conditions was obtained using an enzyme from Candida antarctica (69% release of capsanthin, 44% of lutein from the respective oleoresins). Bile salts were essential for the activity of all commercial enzymes. A novel hydrolase was discovered in the mycelium-free supernatant of submerged cultures of the edible basidiomycete Pleurotus sapidus. The fungal enzyme cleaved several carotenoid esters almost completely and in the absence of bile salts.
KW - Carotenoid esters
KW - Lipase
KW - Marigold
KW - Paprika
KW - Pleurotus sapidus
UR - http://www.scopus.com/inward/record.url?scp=0037426291&partnerID=8YFLogxK
U2 - 10.1016/S0141-0229(03)00020-6
DO - 10.1016/S0141-0229(03)00020-6
M3 - Article
AN - SCOPUS:0037426291
VL - 32
SP - 623
EP - 628
JO - Enzyme and microbial technology
JF - Enzyme and microbial technology
SN - 0141-0229
IS - 5
ER -