TY - JOUR

T1 - Enolases: storage compounds in seeds?

T2 - Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill

AU - Rode, Christina

AU - Gallien, Sébastien

AU - Heintz, Dimitri

AU - Van Dorsselaer, Alain

AU - Braun, Hans Peter

AU - Winkelmann, Traud

N1 - Funding information: Acknowledgments The authors would like to thank Michael Senkler for programming GelMap and for computer-related assistance, Kathrin Lindhorst for her excellent technical assistance in the lab, Dr. Frank Colditz and Jenniffer W. Mwangi for critically reading the manuscript and Varinova for providing Cyclamen plants. The project was funded by the DFG (Deutsche Forschungsgemeinschaft-German Research Foundation).

PY - 2011/1/20

Y1 - 2011/1/20

N2 - Somatic embryogenesis is well established for the economic relevant ornamental crop Cyclamen and thus could supplement the elaborate propagation via seeds. However, the use of somatic embryogenesis for commercial large scale propagation is still limited due to physiological disorders and asynchronous development within emerged embryos. To overcome these problems, profound knowledge of the physiological processes in Cyclamen embryogenesis is essential. Thus, the proteomes of somatic and zygotic embryos were characterised in a comparative approach. Protein separation via two dimensional IEF-SDS PAGE led to a resolution of more than 1,000 protein spots/gel. Overall, 246 proteins were of differential abundance in the two tissues compared. Mass spectrometry analysis of the 300 most abundant protein spots resulted in the identification of 247 proteins, which represent 90 distinct protein species. Fifty-five percent of the 247 proteins belong to only three physiological categories: glycolysis, protein folding and stress response. The latter physiological process was especially predominant in the somatic embryos. Remarkably, the glycolytic enzyme enolase was the protein most frequently detected and thus is supposed to play an important role in Cyclamen embryogenesis. Data are presented that indicate involvement of "small enolases" as storage proteins in Cyclamen. A digital reference map was established via a novel software tool for the web-based presentation of proteome data linked to KEGG and ExPasy protein-databases and both were made publicly available online.

AB - Somatic embryogenesis is well established for the economic relevant ornamental crop Cyclamen and thus could supplement the elaborate propagation via seeds. However, the use of somatic embryogenesis for commercial large scale propagation is still limited due to physiological disorders and asynchronous development within emerged embryos. To overcome these problems, profound knowledge of the physiological processes in Cyclamen embryogenesis is essential. Thus, the proteomes of somatic and zygotic embryos were characterised in a comparative approach. Protein separation via two dimensional IEF-SDS PAGE led to a resolution of more than 1,000 protein spots/gel. Overall, 246 proteins were of differential abundance in the two tissues compared. Mass spectrometry analysis of the 300 most abundant protein spots resulted in the identification of 247 proteins, which represent 90 distinct protein species. Fifty-five percent of the 247 proteins belong to only three physiological categories: glycolysis, protein folding and stress response. The latter physiological process was especially predominant in the somatic embryos. Remarkably, the glycolytic enzyme enolase was the protein most frequently detected and thus is supposed to play an important role in Cyclamen embryogenesis. Data are presented that indicate involvement of "small enolases" as storage proteins in Cyclamen. A digital reference map was established via a novel software tool for the web-based presentation of proteome data linked to KEGG and ExPasy protein-databases and both were made publicly available online.

KW - Differential gel electrophoresis

KW - Embryogenesis

KW - Mass spectrometry

KW - Protein identification

KW - Proteome reference maps

KW - Two-dimensional polyacrylamide gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=79151483037&partnerID=8YFLogxK

U2 - 10.15488/11660

DO - 10.15488/11660

M3 - Article

C2 - 21249422

AN - SCOPUS:79151483037

VL - 75

SP - 305

EP - 319

JO - Plant molecular biology

JF - Plant molecular biology

SN - 0167-4412

IS - 3

ER -