TY - JOUR

T1 - Endothelialization of electrospun polycaprolactone (PCL) small caliber vascular grafts spun from different polymer blends

AU - Pfeiffer, Dagmar

AU - Stefanitsch, C.

AU - Wankhammer, K.

AU - Müller, M.

AU - Dreyer, L.

AU - Krolitzki, B.

AU - Zernetsch, H.

AU - Glasmacher, B.

AU - Lindner, C.

AU - Lass, A.

AU - Schwarz, M.

AU - Muckenauer, W.

AU - Lang-Olip, Ingrid

N1 - German Research Foundation; contract grant number: SFB-TR37-Q2, B4 REBIRTH; contract grant number: EXC 62/1 Government of Styria; contract grant number: A3–22H-2/2011-41 Lower Austria; contract grant number: WST3-T-91/023–2010; LS09–018

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts.

AB - Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts.

KW - Electrospinning

KW - Endothelial cells

KW - Graft

KW - Tissue engineering

KW - Vascular prosthesis

UR - http://www.scopus.com/inward/record.url?scp=84911806556&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.35123

DO - 10.1002/jbm.a.35123

M3 - Article

C2 - 24532056

AN - SCOPUS:84911806556

VL - 102

SP - 4500

EP - 4509

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 12

ER -