TY - JOUR

T1 - Endophytic bacterial communities in in vitro shoot cultures derived from embryonic tissue of hybrid walnut (Juglans × intermedia)

AU - Pham, Ngoc Tuan

AU - Meier-Dinkel, Andreas

AU - Höltken, Aki M.

AU - Quambusch, Mona

AU - Mahnkopp, Felix

AU - Winkelmann, Traud

N1 - Publisher Copyright: © 2017, Springer Science+Business Media Dordrecht. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - A detailed picture of the endophytic bacterial community structure on phylum and genus level in in vitro shoot cultures of sixteen Juglans genotypes including Juglans regia, Juglans nigra and mainly Juglans hybrids was generated by using culture-dependent and culture-independent methods. A total of eight endophytic bacterial strains with a high identity score (97–100%) were isolated from tissue culture shoot material derived from embryonic tissue of Juglans hybrids by the culture-dependent approach. By sequence comparison of a 16S rRNA fragment to the NCBI database, they were identified as Acinetobacter, Brevundimonas, Moraxella, Pseudomonas, and Roseomonas species. The culture-independent approach involved Illumina MiSeq 16S rRNA amplicon sequencing. Five different phyla (Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, and Proteobacteria) were analyzed in more detail. The phylum Proteobacteria including genera such as Brevundimonas spp., Acinetobacter spp. and Moraxella spp. showed the highest relative abundance of endophytic bacteria. Thus, the results of the sequencing approach accorded well with those obtained in the culture-dependent approach. The abundance of Moraxella spp. was correlated with a good micropropagation in the Juglans genotypes under investigation, whereas, Brevundimonas spp. and Acinetobacter spp. were only detected in clones with medium propagation rates. Other bacterial endophytes known as plant growth promoting bacteria such as Pseudomonas spp., Erwinia spp., Burkholderia spp., Pelomonas spp., or Sphingomonas spp. were also found in in vitro cultures of a wide range of Juglans genotypes.

AB - A detailed picture of the endophytic bacterial community structure on phylum and genus level in in vitro shoot cultures of sixteen Juglans genotypes including Juglans regia, Juglans nigra and mainly Juglans hybrids was generated by using culture-dependent and culture-independent methods. A total of eight endophytic bacterial strains with a high identity score (97–100%) were isolated from tissue culture shoot material derived from embryonic tissue of Juglans hybrids by the culture-dependent approach. By sequence comparison of a 16S rRNA fragment to the NCBI database, they were identified as Acinetobacter, Brevundimonas, Moraxella, Pseudomonas, and Roseomonas species. The culture-independent approach involved Illumina MiSeq 16S rRNA amplicon sequencing. Five different phyla (Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, and Proteobacteria) were analyzed in more detail. The phylum Proteobacteria including genera such as Brevundimonas spp., Acinetobacter spp. and Moraxella spp. showed the highest relative abundance of endophytic bacteria. Thus, the results of the sequencing approach accorded well with those obtained in the culture-dependent approach. The abundance of Moraxella spp. was correlated with a good micropropagation in the Juglans genotypes under investigation, whereas, Brevundimonas spp. and Acinetobacter spp. were only detected in clones with medium propagation rates. Other bacterial endophytes known as plant growth promoting bacteria such as Pseudomonas spp., Erwinia spp., Burkholderia spp., Pelomonas spp., or Sphingomonas spp. were also found in in vitro cultures of a wide range of Juglans genotypes.

KW - 16S rRNA amplicon sequencing

KW - Bacterial endophytes

KW - Illumina MiSeq sequencing

KW - In vitro propagation

KW - Juglans hybrids

KW - Juglans × intermedia

KW - Juglans x intermedia

UR - http://www.scopus.com/inward/record.url?scp=85017151042&partnerID=8YFLogxK

U2 - 10.1007/s11240-017-1211-x

DO - 10.1007/s11240-017-1211-x

M3 - Article

AN - SCOPUS:85017151042

VL - 130

SP - 153

EP - 165

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -