Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • B. Rehbock
  • D. Ganßer
  • R. G. Berger

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OriginalspracheEnglisch
Seiten (von - bis)161-165
Seitenumfang5
FachzeitschriftFood chemistry
Jahrgang63
Ausgabenummer2
PublikationsstatusVeröffentlicht - 30 Okt. 1998

Abstract

2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.

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Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings. / Rehbock, B.; Ganßer, D.; Berger, R. G.
in: Food chemistry, Jahrgang 63, Nr. 2, 30.10.1998, S. 161-165.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Rehbock B, Ganßer D, Berger RG. Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings. Food chemistry. 1998 Okt 30;63(2):161-165. doi: 10.1016/S0308-8146(98)00042-9
Rehbock, B. ; Ganßer, D. ; Berger, R. G. / Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings. in: Food chemistry. 1998 ; Jahrgang 63, Nr. 2. S. 161-165.
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abstract = "2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.",
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T1 - Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings

AU - Rehbock, B.

AU - Ganßer, D.

AU - Berger, R. G.

N1 - Funding information: This work was supported by the EU project AIR3-CT94-2060a nd by the Fonds der ChemischenIn dustrie, Frankfurt.

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N2 - 2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.

AB - 2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.

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