Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1-7 |
| Seitenumfang | 7 |
| Fachzeitschrift | Cryobiology |
| Jahrgang | 74 |
| Frühes Online-Datum | 9 Dez. 2016 |
| Publikationsstatus | Veröffentlicht - 1 Feb. 2017 |
Abstract
Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that freezing may be associated with deviations from the physiological epigenetic marks with putative long-term effects on the cells and/or their derivatives. A better understanding of the underlying mechanisms would be beneficial for improving safety and effectiveness of freezing protocols. The purpose of this review is to provide current information regarding epigenetic alterations (DNA methylation and histone modification patterns) associated with cryopreservation.
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in: Cryobiology, Jahrgang 74, 01.02.2017, S. 1-7.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung
}
TY - JOUR
T1 - Effects of cryopreservation on the epigenetic profile of cells
AU - Chatterjee, Anamika
AU - Saha, D.
AU - Niemann, Heiner
AU - Gryshkov, Oleksandr
AU - Glasmacher, Birgit
AU - Hofmann, Nicola Sabine
N1 - Publisher Copyright: © 2016 Elsevier Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that freezing may be associated with deviations from the physiological epigenetic marks with putative long-term effects on the cells and/or their derivatives. A better understanding of the underlying mechanisms would be beneficial for improving safety and effectiveness of freezing protocols. The purpose of this review is to provide current information regarding epigenetic alterations (DNA methylation and histone modification patterns) associated with cryopreservation.
AB - Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that freezing may be associated with deviations from the physiological epigenetic marks with putative long-term effects on the cells and/or their derivatives. A better understanding of the underlying mechanisms would be beneficial for improving safety and effectiveness of freezing protocols. The purpose of this review is to provide current information regarding epigenetic alterations (DNA methylation and histone modification patterns) associated with cryopreservation.
KW - Cellular effects
KW - Cryopreservation
KW - DNA methylation
KW - Histone post-translational modifications
UR - http://www.scopus.com/inward/record.url?scp=85008237797&partnerID=8YFLogxK
U2 - 10.1016/j.cryobiol.2016.12.002
DO - 10.1016/j.cryobiol.2016.12.002
M3 - Article
C2 - 27940283
AN - SCOPUS:85008237797
VL - 74
SP - 1
EP - 7
JO - Cryobiology
JF - Cryobiology
SN - 0011-2240
ER -