TY - JOUR

T1 - Effect of sweet cherry genes PaLACS2 and PaATT1 on cuticle deposition, composition and permeability in Arabidopsis

AU - Declercq, Myriam

AU - Alkio, Merianne

AU - Sprink, Thorben

AU - Schreiber, Lukas

AU - Knoche, Moritz

N1 - Funding Information: We thank Uwe Jonas and Friederike Schröder for technical support and Herbert Geyer and his team for cultivating the Arabidopsis plants. This work was supported in part by grants from the Deutsche Forschungsgemeinschaft (grant numbers AL1373/1-1 and SCHR506/11-1).

PY - 2014/12

Y1 - 2014/12

N2 - The cuticular membrane (CM) of sweet cherry (Prunus avium L.) fruit is severely strained during development. Strain results from a cessation of CM deposition during early development and is possibly caused by a downregulation of genes involved in CM synthesis. The objectives of our study were to investigate the effects of ectopic expression of two sweet cherry genes, PaLACS2 (a putative long-chain acyl-CoA synthetase) and PaATT1 (a putative cytochrome P450 monooxygenase), in Arabidopsis thaliana (L.). Effects on the expression of endogenous LACS2, ATT1 and LACS1 genes, wax and cutin composition, and cuticle permeability were investigated in 13 transgenic lines. Of these, six lines are selected for presentation based on the magnitude of the response. The amount of cutin increased in the PaLACS2 overexpression line C-L-29 and in the complemented lacs2-1 knockout mutant line l-L-14, but overexpression had no effect on cutin composition or wax. Wax deposition decreased in the complemented knockout lines l-L-14 and l-L-21. Overexpressing PaATT1 in A. thaliana line C-A-6 had no significant effect on cutin and wax deposition. In the complemented knockout lines a-A-7 and a-A-12, cutin deposition increased, whereas wax deposition was unaffected. The permeability of the cuticle for water and toluidine blue decreased in the PaLACS2 and PaATT1 complemented knockout lines. The results suggest that (1) PaLACS2 and PaATT1 expressed in A. thaliana are involved in cutin biosynthesis, and (2) their functions are consistent with those of a typical long-chain acyl-CoA synthetase (PaLACS2) and of a cytochrome P450 monooxygenase (PaATT1).

AB - The cuticular membrane (CM) of sweet cherry (Prunus avium L.) fruit is severely strained during development. Strain results from a cessation of CM deposition during early development and is possibly caused by a downregulation of genes involved in CM synthesis. The objectives of our study were to investigate the effects of ectopic expression of two sweet cherry genes, PaLACS2 (a putative long-chain acyl-CoA synthetase) and PaATT1 (a putative cytochrome P450 monooxygenase), in Arabidopsis thaliana (L.). Effects on the expression of endogenous LACS2, ATT1 and LACS1 genes, wax and cutin composition, and cuticle permeability were investigated in 13 transgenic lines. Of these, six lines are selected for presentation based on the magnitude of the response. The amount of cutin increased in the PaLACS2 overexpression line C-L-29 and in the complemented lacs2-1 knockout mutant line l-L-14, but overexpression had no effect on cutin composition or wax. Wax deposition decreased in the complemented knockout lines l-L-14 and l-L-21. Overexpressing PaATT1 in A. thaliana line C-A-6 had no significant effect on cutin and wax deposition. In the complemented knockout lines a-A-7 and a-A-12, cutin deposition increased, whereas wax deposition was unaffected. The permeability of the cuticle for water and toluidine blue decreased in the PaLACS2 and PaATT1 complemented knockout lines. The results suggest that (1) PaLACS2 and PaATT1 expressed in A. thaliana are involved in cutin biosynthesis, and (2) their functions are consistent with those of a typical long-chain acyl-CoA synthetase (PaLACS2) and of a cytochrome P450 monooxygenase (PaATT1).

KW - Aberrant Induction of Type Three 1 (ATT1)

KW - Cutin

KW - Ectopic expression

KW - Long-chain acyl-CoA synthetase 2 (LACS2)

KW - Prunus avium

KW - Wax

UR - http://www.scopus.com/inward/record.url?scp=85027937547&partnerID=8YFLogxK

U2 - 10.1007/s11295-014-0791-4

DO - 10.1007/s11295-014-0791-4

M3 - Article

AN - SCOPUS:85027937547

VL - 10

SP - 1711

EP - 1721

JO - Tree Genetics and Genomes

JF - Tree Genetics and Genomes

SN - 1614-2942

IS - 6

ER -