TY - JOUR

T1 - Effect of cytokinins and light quality on adventitious shoot regeneration from leaflet explants of peanut (Arachis hypogaea)

AU - Assou, Juvenal

AU - Bethge, Hans

AU - Wamhoff, David

AU - Winkelmann, Traud

N1 - Funding Information: This work was funded by the Federal Ministry of Education and Research (BMBF, grant number 031B0348). The authors thank Ewa Schneider for excellent technical assistance and Thomas Reinard, Institute of Plant Genetics at Leibniz University Hannover, Germany, as well Prof. Imdadul Hoque, Institute of Biological Science at the University of Dhaka, Bangladesh, as well as the gene bank of the United States Department of Agriculture (USDA) for provision of seed materials.

PY - 2023

Y1 - 2023

N2 - Plant tissue culture and novel breeding techniques such as genome editing in the economically important crop Arachis hypogaea constitute potential for genetic improvement. Therefore, in this study, high-frequency in vitro regeneration via formation of adventitious shoots was described for different A. hypogaea lines. The leaflets of the primary leaves of 5-day-old seedlings were cultured for 16 weeks on Murashige and Skoog’s medium supplemented with equimolar concentrations (22.19 µM) of the cytokinins 6-benzylaminopurine (BAP); thidiazuron (TDZ); zeatin (Zea) or meta-topolin (MT), which was reduced to 7.40 µM after eight weeks plus 2.3 µM kinetin. Highest shoot regeneration percentages of 89.1% and 81.3% for BAP and TDZ containing media, respectively, were achieved in the line ’Jimmy’s pride’ with the maximum shoot number obtained on BAP. The regeneration percentages for three further lines were lower than 50%. Furthermore, the effect of different light qualities, applied using LEDs, and compared to fluorescent lamps was investigated. Shoot regeneration percentages did not improve significantly under the different LEDs, instead a pronounced effect on the regeneration efficiency of the culture temperature was observed which differed among the light treatments. However, fluorescent lamps can be replaced by red or blue LEDs without negative effects on shoot regeneration.

AB - Plant tissue culture and novel breeding techniques such as genome editing in the economically important crop Arachis hypogaea constitute potential for genetic improvement. Therefore, in this study, high-frequency in vitro regeneration via formation of adventitious shoots was described for different A. hypogaea lines. The leaflets of the primary leaves of 5-day-old seedlings were cultured for 16 weeks on Murashige and Skoog’s medium supplemented with equimolar concentrations (22.19 µM) of the cytokinins 6-benzylaminopurine (BAP); thidiazuron (TDZ); zeatin (Zea) or meta-topolin (MT), which was reduced to 7.40 µM after eight weeks plus 2.3 µM kinetin. Highest shoot regeneration percentages of 89.1% and 81.3% for BAP and TDZ containing media, respectively, were achieved in the line ’Jimmy’s pride’ with the maximum shoot number obtained on BAP. The regeneration percentages for three further lines were lower than 50%. Furthermore, the effect of different light qualities, applied using LEDs, and compared to fluorescent lamps was investigated. Shoot regeneration percentages did not improve significantly under the different LEDs, instead a pronounced effect on the regeneration efficiency of the culture temperature was observed which differed among the light treatments. However, fluorescent lamps can be replaced by red or blue LEDs without negative effects on shoot regeneration.

KW - In vitro culture

KW - light-emitting diodes

KW - organogenesis

KW - plant growth regulators

KW - temperature

KW - tubular fluorescent lamps

UR - http://www.scopus.com/inward/record.url?scp=85145502996&partnerID=8YFLogxK

U2 - 10.1080/14620316.2022.2160382

DO - 10.1080/14620316.2022.2160382

M3 - Article

AN - SCOPUS:85145502996

VL - 98

SP - 508

EP - 525

JO - Journal of Horticultural Science and Biotechnology

JF - Journal of Horticultural Science and Biotechnology

SN - 1462-0316

IS - 4

ER -