TY - JOUR

T1 - Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases

AU - Hutin, Mathilde

AU - Césari, Stella

AU - Chalvon, Véronique

AU - Michel, Corinne

AU - Tran, Tuan Tu

AU - Boch, Jens

AU - Koebnik, Ralf

AU - Szurek, Boris

AU - Kroj, Thomas

N1 - Funding Information: We thank Loic Fontaine and Christophe Tertois for technical assistance. This work was supported by a grant from GRiSP (MENERGEP New Frontier program), Agropolis Foundation (project 0802-023) and the Genoplante programme (Project ?Interaction Rice Magnaporthe?). M.H. was supported by doctoral fellowships awarded by the Minist?re de l'Enseignement sup?rieur et de la Recherche (MESR). S.C. was supported by an INRA CJS grant (?contrat jeune scientifique?). TT is supported by a doctoral fellowship awarded by the Erasmus Mundus Action?2 PANACEA program of the European Community and a scholarship from The Vietnam International Education Development (VIED). This work benefited from interactions promoted by COST Action FA 1208 (http://www.cost-sustain.org). Publisher Copyright: © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Bacterial blight (BB) and bacterial leaf streak (BLS) are important diseases in Oryza sativa caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively. In both bacteria, transcription activator-like (TAL) effectors are major virulence determinants that act by transactivating host genes downstream of effector-binding elements (EBEs) bound in a sequence-specific manner. Resistance to Xoo is mostly related to the action of TAL effectors, either by polymorphisms that prevent the induction of susceptibility (S) genes or by executor (R) genes with EBEs embedded in their promoter, and that induce cell death and resistance. For Xoc, no resistance sources are known in rice. Here, we investigated whether the recognition of effectors by nucleotide binding and leucine-rich repeat domain immune receptors (NLRs), the most widespread resistance mechanism in plants, is also able to stop BB and BLS. In one instance, transgenic rice lines harboring the AVR1-CO39 effector gene from the rice blast fungus Magnaporthe oryzae, under the control of an inducible promoter, were challenged with transgenic Xoo and Xoc strains carrying a TAL effector designed to transactivate the inducible promoter. This induced AVR1-CO39 expression and triggered BB and BLS resistance when the corresponding Pi-CO39 resistance locus was present. In a second example, the transactivation of an auto-active NLR by Xoo-delivered designer TAL effectors resulted in BB resistance, demonstrating that NLR-triggered immune responses efficiently control Xoo. This forms the foundation for future BB and BLS disease control strategies, whereupon endogenous TAL effectors will target synthetic promoter regions of Avr or NLR executor genes.

AB - Bacterial blight (BB) and bacterial leaf streak (BLS) are important diseases in Oryza sativa caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively. In both bacteria, transcription activator-like (TAL) effectors are major virulence determinants that act by transactivating host genes downstream of effector-binding elements (EBEs) bound in a sequence-specific manner. Resistance to Xoo is mostly related to the action of TAL effectors, either by polymorphisms that prevent the induction of susceptibility (S) genes or by executor (R) genes with EBEs embedded in their promoter, and that induce cell death and resistance. For Xoc, no resistance sources are known in rice. Here, we investigated whether the recognition of effectors by nucleotide binding and leucine-rich repeat domain immune receptors (NLRs), the most widespread resistance mechanism in plants, is also able to stop BB and BLS. In one instance, transgenic rice lines harboring the AVR1-CO39 effector gene from the rice blast fungus Magnaporthe oryzae, under the control of an inducible promoter, were challenged with transgenic Xoo and Xoc strains carrying a TAL effector designed to transactivate the inducible promoter. This induced AVR1-CO39 expression and triggered BB and BLS resistance when the corresponding Pi-CO39 resistance locus was present. In a second example, the transactivation of an auto-active NLR by Xoo-delivered designer TAL effectors resulted in BB resistance, demonstrating that NLR-triggered immune responses efficiently control Xoo. This forms the foundation for future BB and BLS disease control strategies, whereupon endogenous TAL effectors will target synthetic promoter regions of Avr or NLR executor genes.

UR - http://www.scopus.com/inward/record.url?scp=84982299739&partnerID=8YFLogxK

U2 - 10.1111/tpj.13231

DO - 10.1111/tpj.13231

M3 - Article

C2 - 27289079

AN - SCOPUS:84982299739

VL - 88

SP - 43

EP - 55

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 1

ER -