Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 940-947 |
| Seitenumfang | 8 |
| Fachzeitschrift | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Jahrgang | 100 B |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 30 Jan. 2012 |
| Extern publiziert | Ja |
Abstract
For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Jahrgang 100 B, Nr. 4, 30.01.2012, S. 940-947.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Directing neuronal cell growth on implant material surfaces by microstructuring
AU - Reich, Uta
AU - Fadeeva, Elena
AU - Warnecke, Athanasia
AU - Paasche, Gerrit
AU - Müller, Peter
AU - Chichkov, Boris
AU - Stöver, Timo
AU - Lenarz, Thomas
AU - Reuter, Günter
PY - 2012/1/30
Y1 - 2012/1/30
N2 - For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.
AB - For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.
KW - cochlear implant electrode
KW - guided neuronal outgrowth
KW - PC-12 cells
KW - spiral ganglion neurons
KW - surface microstructure
UR - http://www.scopus.com/inward/record.url?scp=84859603694&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.32656
DO - 10.1002/jbm.b.32656
M3 - Article
C2 - 22287482
AN - SCOPUS:84859603694
VL - 100 B
SP - 940
EP - 947
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
SN - 1552-4973
IS - 4
ER -