Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | XXIV International Eucarpia Symposium Section Ornamentals |
| Untertitel | Ornamental Breeding Worldwide |
| Herausgeber (Verlag) | International Society for Horticultural Science |
| Seiten | 201-208 |
| Seitenumfang | 8 |
| ISBN (Print) | 9789066054172 |
| Publikationsstatus | Veröffentlicht - 1 Sept. 2012 |
Publikationsreihe
| Name | Acta Horticulturae |
|---|---|
| Band | 953 |
| ISSN (Print) | 0567-7572 |
Abstract
In situ PCR is a technique, which allows detection of specific nucleic acid sequences in particular cells and tissues. In plants, in situ RT-PCR is widely used in localisation of specific genes, including MADS-box, and other function-specific genes of floral buds and other organs. Roses are one of the most economically important ornamental plants, and a number of cultivars have been selected based on flower traits, such as petal form, colour, and number. Of particular interest to floriculture researchers and breeders is flower colour and petal number, which is connected to MADS-box genes expression. In this report, we present a convenient protocol for the localisation of transcript expression in Rosa hybrida. The protocol is more appealing because of a high sensitivity for the in situ RT-PCR reaction and its speed. We demonstrated that a two-step reaction can be completed in two days, and a one-step reaction with fluorescent nucleotide used as a label can be completed in one day. Our results showed that a normal RT-PCR reaction performed directly on tissue showed a high specific expression of examined genes in rose organs.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Gartenbau
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XXIV International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide. International Society for Horticultural Science, 2012. S. 201-208 (Acta Horticulturae; Band 953).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Beitrag in Buch/Sammelwerk › Forschung › Peer-Review
}
TY - CHAP
T1 - Direct in situ RT-PCR as a useful tool in localisation of abundant and organ-specific genes expressed in Rosa hybrida
AU - Jedrzejuk, A.
AU - Mibus, H.
AU - Serek, M.
PY - 2012/9/1
Y1 - 2012/9/1
N2 - In situ PCR is a technique, which allows detection of specific nucleic acid sequences in particular cells and tissues. In plants, in situ RT-PCR is widely used in localisation of specific genes, including MADS-box, and other function-specific genes of floral buds and other organs. Roses are one of the most economically important ornamental plants, and a number of cultivars have been selected based on flower traits, such as petal form, colour, and number. Of particular interest to floriculture researchers and breeders is flower colour and petal number, which is connected to MADS-box genes expression. In this report, we present a convenient protocol for the localisation of transcript expression in Rosa hybrida. The protocol is more appealing because of a high sensitivity for the in situ RT-PCR reaction and its speed. We demonstrated that a two-step reaction can be completed in two days, and a one-step reaction with fluorescent nucleotide used as a label can be completed in one day. Our results showed that a normal RT-PCR reaction performed directly on tissue showed a high specific expression of examined genes in rose organs.
AB - In situ PCR is a technique, which allows detection of specific nucleic acid sequences in particular cells and tissues. In plants, in situ RT-PCR is widely used in localisation of specific genes, including MADS-box, and other function-specific genes of floral buds and other organs. Roses are one of the most economically important ornamental plants, and a number of cultivars have been selected based on flower traits, such as petal form, colour, and number. Of particular interest to floriculture researchers and breeders is flower colour and petal number, which is connected to MADS-box genes expression. In this report, we present a convenient protocol for the localisation of transcript expression in Rosa hybrida. The protocol is more appealing because of a high sensitivity for the in situ RT-PCR reaction and its speed. We demonstrated that a two-step reaction can be completed in two days, and a one-step reaction with fluorescent nucleotide used as a label can be completed in one day. Our results showed that a normal RT-PCR reaction performed directly on tissue showed a high specific expression of examined genes in rose organs.
KW - Abundant genes
KW - Immunolocalization
KW - In situ RT-PCR
KW - Rosa hybrida
UR - http://www.scopus.com/inward/record.url?scp=84870024648&partnerID=8YFLogxK
U2 - 10.17660/actahortic.2012.953.28
DO - 10.17660/actahortic.2012.953.28
M3 - Contribution to book/anthology
AN - SCOPUS:84870024648
SN - 9789066054172
T3 - Acta Horticulturae
SP - 201
EP - 208
BT - XXIV International Eucarpia Symposium Section Ornamentals
PB - International Society for Horticultural Science
ER -