Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 148-157 |
| Seitenumfang | 10 |
| Fachzeitschrift | Cryo-letters |
| Jahrgang | 32 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 1 März 2011 |
Abstract
Fourier transform infrared spectroscopy (FTIR) and cryomicroscopy were used to study the effects of dimethyl sulfoxide and ethylene glycol on cell pellets of human pulmonary microvascular endothelial cells during freezing from 4°C to -60°C at 1°C/min. FTIR analysis showed that membranes undergo a phase change in the presence of cryoprotective agents (CPAs) which was not observed in the absence of CPAs. Cryomicroscopy revealed the formation of intracellular ice and concomitant cell volume changes. Intracellular ice was detected in the majority of the cells both in the presence and absence of CPAs. Membrane phase changes were found to be most pronounced at intermediate concentrations of cryoprotective agents; for dimethyl sulfoxide at around 1 M and for ethylene glycol at around 1.5 M. At those concentrations cell survival after thawing exhibited a maximum. The results indicate that CPAs promote rather than prevent cell dehydration during freezing.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Anatomie
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
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in: Cryo-letters, Jahrgang 32, Nr. 2, 01.03.2011, S. 148-157.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Dimethyl sulfoxide and ethylene glycol promote membrane phase change during cryopreservation
AU - Spindler, Ralf
AU - Wolkers, Willem F.
AU - Glasmacher, Birgit
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Fourier transform infrared spectroscopy (FTIR) and cryomicroscopy were used to study the effects of dimethyl sulfoxide and ethylene glycol on cell pellets of human pulmonary microvascular endothelial cells during freezing from 4°C to -60°C at 1°C/min. FTIR analysis showed that membranes undergo a phase change in the presence of cryoprotective agents (CPAs) which was not observed in the absence of CPAs. Cryomicroscopy revealed the formation of intracellular ice and concomitant cell volume changes. Intracellular ice was detected in the majority of the cells both in the presence and absence of CPAs. Membrane phase changes were found to be most pronounced at intermediate concentrations of cryoprotective agents; for dimethyl sulfoxide at around 1 M and for ethylene glycol at around 1.5 M. At those concentrations cell survival after thawing exhibited a maximum. The results indicate that CPAs promote rather than prevent cell dehydration during freezing.
AB - Fourier transform infrared spectroscopy (FTIR) and cryomicroscopy were used to study the effects of dimethyl sulfoxide and ethylene glycol on cell pellets of human pulmonary microvascular endothelial cells during freezing from 4°C to -60°C at 1°C/min. FTIR analysis showed that membranes undergo a phase change in the presence of cryoprotective agents (CPAs) which was not observed in the absence of CPAs. Cryomicroscopy revealed the formation of intracellular ice and concomitant cell volume changes. Intracellular ice was detected in the majority of the cells both in the presence and absence of CPAs. Membrane phase changes were found to be most pronounced at intermediate concentrations of cryoprotective agents; for dimethyl sulfoxide at around 1 M and for ethylene glycol at around 1.5 M. At those concentrations cell survival after thawing exhibited a maximum. The results indicate that CPAs promote rather than prevent cell dehydration during freezing.
KW - Cryomicroscopy
KW - Cryopreservation
KW - Dehydration
KW - Dimethyl sulfoxide
KW - Ethylene glycol
KW - FTIR
UR - http://www.scopus.com/inward/record.url?scp=79957858727&partnerID=8YFLogxK
M3 - Article
C2 - 21766144
AN - SCOPUS:79957858727
VL - 32
SP - 148
EP - 157
JO - Cryo-letters
JF - Cryo-letters
SN - 0143-2044
IS - 2
ER -