Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 493-503 |
| Seitenumfang | 11 |
| Fachzeitschrift | Cryo-letters |
| Jahrgang | 31 |
| Ausgabenummer | 6 |
| Publikationsstatus | Veröffentlicht - Nov. 2010 |
Abstract
Cryopreservation is a convenient method for long-term preservation of natural and engineered tissues in regenerative medicine. Homogeneous loading of tissues with CPAs, however, forms one of the major hurdles in tissue cryopreservation. In this study, computer tomography (CT) as a non-invasive imaging method was used to determine the effective diffusion of Me2SO in tissue-engineered collagen scaffolds. The dimensions of the scaffolds were 30 × 30 × 10mm3 with a homogeneous pore size of 100 μm and a porosity of 98%. CT images were acquired after equilibrating the scaffolds in phosphate buffered saline (PBS) and transferring them directly in 10% (v/v) Me2SO. The Me2SO loading process of the scaffold could thus be measured and visualized in real time. The experimental data were fitted using a diffusion equation. The calculated effective diffusion constant for Me2SO in the PBS loaded scaffold was determined from experimental diffusion studies to be 2.4 × 10-6 cm2/s at 207deg;C.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Anatomie
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Cryo-letters, Jahrgang 31, Nr. 6, 11.2010, S. 493-503.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Diffusion of dimethyl sulfoxide in tissue engineered collagen scaffolds visualized by computer tomography
AU - Bernemann, Inga
AU - Manuchehrabadi, Navid
AU - Spindler, Ralf
AU - Choi, Jeunghwan
AU - Wolkers, Wim F.
AU - Bischof, John C.
AU - Glasmacher, Birgit
PY - 2010/11
Y1 - 2010/11
N2 - Cryopreservation is a convenient method for long-term preservation of natural and engineered tissues in regenerative medicine. Homogeneous loading of tissues with CPAs, however, forms one of the major hurdles in tissue cryopreservation. In this study, computer tomography (CT) as a non-invasive imaging method was used to determine the effective diffusion of Me2SO in tissue-engineered collagen scaffolds. The dimensions of the scaffolds were 30 × 30 × 10mm3 with a homogeneous pore size of 100 μm and a porosity of 98%. CT images were acquired after equilibrating the scaffolds in phosphate buffered saline (PBS) and transferring them directly in 10% (v/v) Me2SO. The Me2SO loading process of the scaffold could thus be measured and visualized in real time. The experimental data were fitted using a diffusion equation. The calculated effective diffusion constant for Me2SO in the PBS loaded scaffold was determined from experimental diffusion studies to be 2.4 × 10-6 cm2/s at 207deg;C.
AB - Cryopreservation is a convenient method for long-term preservation of natural and engineered tissues in regenerative medicine. Homogeneous loading of tissues with CPAs, however, forms one of the major hurdles in tissue cryopreservation. In this study, computer tomography (CT) as a non-invasive imaging method was used to determine the effective diffusion of Me2SO in tissue-engineered collagen scaffolds. The dimensions of the scaffolds were 30 × 30 × 10mm3 with a homogeneous pore size of 100 μm and a porosity of 98%. CT images were acquired after equilibrating the scaffolds in phosphate buffered saline (PBS) and transferring them directly in 10% (v/v) Me2SO. The Me2SO loading process of the scaffold could thus be measured and visualized in real time. The experimental data were fitted using a diffusion equation. The calculated effective diffusion constant for Me2SO in the PBS loaded scaffold was determined from experimental diffusion studies to be 2.4 × 10-6 cm2/s at 207deg;C.
KW - Chemical loading
KW - Collagen scaffolds
KW - Computer tomography
KW - CPA diffusion
KW - Freezing of tissue engineered products
KW - Visualization
UR - http://www.scopus.com/inward/record.url?scp=79951534251&partnerID=8YFLogxK
M3 - Article
C2 - 21410018
AN - SCOPUS:79951534251
VL - 31
SP - 493
EP - 503
JO - Cryo-letters
JF - Cryo-letters
SN - 0143-2044
IS - 6
ER -