TY - JOUR

T1 - Differential expression of Arabidopsis sulfurtransferases under various growth conditions

AU - Bartels, Andrea

AU - Mock, Hans Peter

AU - Papenbrock, Jutta

N1 - Funding information: We thank Pamela von Trzebiatowski and Julia Volker for their excellent technical assistance and the gardeners for growing the plants. The work was financially supported by the Deutsche Forschungsgemeinschaft (PA 764/1-4, SCHM 307/15-3).

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyse the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Arabidopsis thaliana 20 Str proteins have been identified and grouped according to sequence homology. To investigate their respective in vivo function, Arabidopsis plants were grown in sterile hydroponic cultures at different sulfate (50, 500, and 1500 μM) and phosphate (0.1 and 1 mM) concentrations, and in medium supplemented with 1 mM thiosulfate. Northern blot analysis revealed the differential expression of the Str investigated. Thiosulfate Str activity was significantly increased at low sulfate concentrations in the medium. The Str mRNA levels were highly dependent on the developmental stage of the Arabidopsis plants. The expression of most Str analysed increased with progressing plant age in parallel with increasing 3-mercaptopyruvate and thiosulfate Str activities. The Str investigated were differentially expressed in a light/dark cycle whereas Str enzyme activities were not affected by the light conditions. The results indicate that each Str is regulated in a different way and plays an individual specific role in the plant metabolism.

AB - Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyse the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Arabidopsis thaliana 20 Str proteins have been identified and grouped according to sequence homology. To investigate their respective in vivo function, Arabidopsis plants were grown in sterile hydroponic cultures at different sulfate (50, 500, and 1500 μM) and phosphate (0.1 and 1 mM) concentrations, and in medium supplemented with 1 mM thiosulfate. Northern blot analysis revealed the differential expression of the Str investigated. Thiosulfate Str activity was significantly increased at low sulfate concentrations in the medium. The Str mRNA levels were highly dependent on the developmental stage of the Arabidopsis plants. The expression of most Str analysed increased with progressing plant age in parallel with increasing 3-mercaptopyruvate and thiosulfate Str activities. The Str investigated were differentially expressed in a light/dark cycle whereas Str enzyme activities were not affected by the light conditions. The results indicate that each Str is regulated in a different way and plays an individual specific role in the plant metabolism.

KW - 3-Mercaptopyruvate

KW - Arabidopsis

KW - Nutrition

KW - Thiosulfate

UR - http://www.scopus.com/inward/record.url?scp=34247349068&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2007.02.005

DO - 10.1016/j.plaphy.2007.02.005

M3 - Article

C2 - 17408957

AN - SCOPUS:34247349068

VL - 45

SP - 178

EP - 187

JO - Plant physiology and biochemistry

JF - Plant physiology and biochemistry

SN - 0981-9428

IS - 3-4

ER -