Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 830-832 |
| Seitenumfang | 3 |
| Fachzeitschrift | Fresenius' Journal of Analytical Chemistry |
| Jahrgang | 360 |
| Ausgabenummer | 7-8 |
| Publikationsstatus | Veröffentlicht - Apr. 1998 |
Abstract
The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Fresenius' Journal of Analytical Chemistry, Jahrgang 360, Nr. 7-8, 04.1998, S. 830-832.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Different detection systems for the HPLC analysis of oxylipins, by-products of the enzymatic generation of 2E-hexenal
AU - Rehbock, Bettina
AU - Ganßer, Dietmar
AU - Berger, Ralf G.
PY - 1998/4
Y1 - 1998/4
N2 - The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity.
AB - The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity.
UR - http://www.scopus.com/inward/record.url?scp=25144477082&partnerID=8YFLogxK
U2 - 10.1007/s002160050820
DO - 10.1007/s002160050820
M3 - Article
AN - SCOPUS:25144477082
VL - 360
SP - 830
EP - 832
JO - Fresenius' Journal of Analytical Chemistry
JF - Fresenius' Journal of Analytical Chemistry
SN - 0937-0633
IS - 7-8
ER -