Different detection systems for the HPLC analysis of oxylipins, by-products of the enzymatic generation of 2E-hexenal

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Bettina Rehbock
  • Dietmar Ganßer
  • Ralf G. Berger

Organisationseinheiten

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Details

OriginalspracheEnglisch
Seiten (von - bis)830-832
Seitenumfang3
FachzeitschriftFresenius' Journal of Analytical Chemistry
Jahrgang360
Ausgabenummer7-8
PublikationsstatusVeröffentlicht - Apr. 1998

Abstract

The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biochemie

Zitieren

Different detection systems for the HPLC analysis of oxylipins, by-products of the enzymatic generation of 2E-hexenal. / Rehbock, Bettina; Ganßer, Dietmar; Berger, Ralf G.
in: Fresenius' Journal of Analytical Chemistry, Jahrgang 360, Nr. 7-8, 04.1998, S. 830-832.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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abstract = "The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity.",
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Download

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T1 - Different detection systems for the HPLC analysis of oxylipins, by-products of the enzymatic generation of 2E-hexenal

AU - Rehbock, Bettina

AU - Ganßer, Dietmar

AU - Berger, Ralf G.

PY - 1998/4

Y1 - 1998/4

N2 - The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity.

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