TY - JOUR

T1 - Dextrans, Pullulan and Lentinan, New Scaffold Materials for Use as Hydrogels in Tissue Engineering

AU - Lienig, Pascal

AU - Banerjee, Samhita

AU - Kenneweg, Franziska

AU - Thum, Thomas

AU - Kirschning, Andreas

AU - Dräger, Gerald

N1 - Funding Information: The work was funded by the Deutsche Forschungsgemeinschaft (Cluster of Excellence REBIRTH; “From Regenerative Biology to Reconstructive Therapy” EXC 62). Additionally, this work has been carried out as an integral part of the BIOFABRICATION FOR NIFE Initiative, which is financially supported by the ministry of Lower Saxony and the Volkswagen Stiftung (NIFE is the Lower Saxony Center for Biomedical Engineering, Implant Research and Development, a joint translational research center of the Hannover Medical School, the Leibniz Universität Hannover, the University of Veterinary Medicine Hannover and the Laser Center Hannover. Open Access funding enabled and organized by Projekt DEAL.

PY - 2024/4/16

Y1 - 2024/4/16

N2 - The development of hydrogels based on dextrans, pullulan and lentinan to be used in biomedical applications including tissue engineering is reported. Despite the fact that selected polysaccharides such as hyaluronic acid are well established, little is known, how these polysaccharides can be chemically modified to create hydrogels under controlled conditions. In this study we present a small library of chemically modified polysaccharides which are used for a divergent approach to achieve biomedical relevant hydrogels. In this case the crosslinking is based on thio ether formation between thiol modified donor and vinylsulfone or maleimide modified acceptor components. Successful synthesis of the linker systems and coupling at the polysaccharides, hydrogel formation takes place under physiological conditions. We extended the study by coupling small molecules like adhesion factors for increasing cell compatibility as well as a dye for further studies. The different hydrogels were studied to their rheological properties, water uptake, their permeability, biodegrability and their cytotoxicity.

AB - The development of hydrogels based on dextrans, pullulan and lentinan to be used in biomedical applications including tissue engineering is reported. Despite the fact that selected polysaccharides such as hyaluronic acid are well established, little is known, how these polysaccharides can be chemically modified to create hydrogels under controlled conditions. In this study we present a small library of chemically modified polysaccharides which are used for a divergent approach to achieve biomedical relevant hydrogels. In this case the crosslinking is based on thio ether formation between thiol modified donor and vinylsulfone or maleimide modified acceptor components. Successful synthesis of the linker systems and coupling at the polysaccharides, hydrogel formation takes place under physiological conditions. We extended the study by coupling small molecules like adhesion factors for increasing cell compatibility as well as a dye for further studies. The different hydrogels were studied to their rheological properties, water uptake, their permeability, biodegrability and their cytotoxicity.

KW - click chemistry

KW - dextran

KW - hydrogels

KW - lentinan

KW - pullulan

UR - http://www.scopus.com/inward/record.url?scp=85186547626&partnerID=8YFLogxK

U2 - 10.1002/chem.202303843

DO - 10.1002/chem.202303843

M3 - Article

AN - SCOPUS:85186547626

VL - 30

JO - Chemistry - a European journal

JF - Chemistry - a European journal

SN - 0947-6539

IS - 22

M1 - e202303843

ER -