TY - GEN

T1 - Development of the WagRhSNP AXIOM SNP array based on sequences from tetraploid cut roses and garden roses

AU - Smulders, M. J.M.

AU - Voorrips, R. E.

AU - Esselink, G. D.

AU - Santos Leonardo, T. M.

AU - Vant' Westende, W. P.C.

AU - Vukosavljev, M.

AU - Koning-Boucoiran, C. F.S.

AU - Van De Weg, W. E.

AU - Arens, P.

AU - Schulz, D.

AU - Debener, T.

AU - Bellon, L.

AU - Mittmann, M.

AU - Pirani, A.

AU - Webster, T.

AU - Brew, F.

AU - Cox, P.

AU - Maliepaard, C.

N1 - Publisher Copyright: © 2015, International Society for Horticultural Science. All rights reserved.

PY - 2015/1/25

Y1 - 2015/1/25

N2 - Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose.

AB - Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose.

KW - Genotyping

KW - Rosa hybrida

KW - Rose

KW - SNP

KW - Tetraploid

UR - http://www.scopus.com/inward/record.url?scp=84928109571&partnerID=8YFLogxK

U2 - 10.17660/ActaHortic.2015.1064.20

DO - 10.17660/ActaHortic.2015.1064.20

M3 - Conference contribution

AN - SCOPUS:84928109571

T3 - Acta Horticulturae

SP - 177

EP - 184

BT - VI International Symposium on Rose Research and Cultivation

A2 - Debener, T.

A2 - Linde, M.

PB - International Society for Horticultural Science

ER -