Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 152-159 |
| Seitenumfang | 8 |
| Fachzeitschrift | Scientia horticulturae |
| Jahrgang | 235 |
| Frühes Online-Datum | 9 März 2018 |
| Publikationsstatus | Veröffentlicht - 17 Mai 2018 |
Abstract
African nightshade is an indigenous leafy vegetable in Eastern and Western Africa that is valued for its high nutrient content and potential to generate income. Efforts to improve this crop have been hampered by limited information available on intra- and interspecific genetic diversity. Here, we present data on the development and application of polymorphic simple sequence repeat (SSR) markers in a broad collection of entries (gene bank accessions, landraces from farmers, breeding lines and commercial cultivars) from two species of African nightshades, Solanum scabrum (Mill.) and S. villosum (Mill.). Next generation sequencing information was used to develop 16 polymorphic SSR markers that revealed genetic diversity within and between the 54 entries collected from various geographic locations in Sub-Saharan Africa. High levels of polymorphism were detected within entries, indicating gene flow between entries and/or a low intensity of selection during development of breeding lines and improved cultivars. As a consequence, they have retained sufficient variation for further selection within the improved germplasm. The two species were clearly distinct, and S. scabrum was found to be less diverse compared to S. villosum. Furthermore, some entries were identified as probably misclassified based on morphological markers as they can clearly be placed within the gene pool of either S. scabrum or S. villosum using the SSR data. In addition to the diversity studies, we determined the genome size and ploidy of a selection of 29 entries helpful for the design of future NGS-based marker projects. All entries of S. scabrum were found to be hexaploid, whereas entries of S. villosum were tetraploid with a haploid genome size of all samples slightly varying by approximately 1 pg. Finally, we also determined pollen viability parameters, which differed significantly between the two species but did not correlate to any of the other parameters measured.
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in: Scientia horticulturae, Jahrgang 235, 17.05.2018, S. 152-159.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Development of next-generation sequencing (NGS)-based SSRs in African nightshades
T2 - Tools for analyzing genetic diversity for conservation and breeding
AU - Ronoh, Regina
AU - Linde, Marcus
AU - Winkelmann, Traud
AU - Abukutsa-Onyango, Mary
AU - Dinssa, Fekadu Fufa
AU - Debener, Thomas
N1 - Publisher Copyright: © 2018 Elsevier B.V. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/5/17
Y1 - 2018/5/17
N2 - African nightshade is an indigenous leafy vegetable in Eastern and Western Africa that is valued for its high nutrient content and potential to generate income. Efforts to improve this crop have been hampered by limited information available on intra- and interspecific genetic diversity. Here, we present data on the development and application of polymorphic simple sequence repeat (SSR) markers in a broad collection of entries (gene bank accessions, landraces from farmers, breeding lines and commercial cultivars) from two species of African nightshades, Solanum scabrum (Mill.) and S. villosum (Mill.). Next generation sequencing information was used to develop 16 polymorphic SSR markers that revealed genetic diversity within and between the 54 entries collected from various geographic locations in Sub-Saharan Africa. High levels of polymorphism were detected within entries, indicating gene flow between entries and/or a low intensity of selection during development of breeding lines and improved cultivars. As a consequence, they have retained sufficient variation for further selection within the improved germplasm. The two species were clearly distinct, and S. scabrum was found to be less diverse compared to S. villosum. Furthermore, some entries were identified as probably misclassified based on morphological markers as they can clearly be placed within the gene pool of either S. scabrum or S. villosum using the SSR data. In addition to the diversity studies, we determined the genome size and ploidy of a selection of 29 entries helpful for the design of future NGS-based marker projects. All entries of S. scabrum were found to be hexaploid, whereas entries of S. villosum were tetraploid with a haploid genome size of all samples slightly varying by approximately 1 pg. Finally, we also determined pollen viability parameters, which differed significantly between the two species but did not correlate to any of the other parameters measured.
AB - African nightshade is an indigenous leafy vegetable in Eastern and Western Africa that is valued for its high nutrient content and potential to generate income. Efforts to improve this crop have been hampered by limited information available on intra- and interspecific genetic diversity. Here, we present data on the development and application of polymorphic simple sequence repeat (SSR) markers in a broad collection of entries (gene bank accessions, landraces from farmers, breeding lines and commercial cultivars) from two species of African nightshades, Solanum scabrum (Mill.) and S. villosum (Mill.). Next generation sequencing information was used to develop 16 polymorphic SSR markers that revealed genetic diversity within and between the 54 entries collected from various geographic locations in Sub-Saharan Africa. High levels of polymorphism were detected within entries, indicating gene flow between entries and/or a low intensity of selection during development of breeding lines and improved cultivars. As a consequence, they have retained sufficient variation for further selection within the improved germplasm. The two species were clearly distinct, and S. scabrum was found to be less diverse compared to S. villosum. Furthermore, some entries were identified as probably misclassified based on morphological markers as they can clearly be placed within the gene pool of either S. scabrum or S. villosum using the SSR data. In addition to the diversity studies, we determined the genome size and ploidy of a selection of 29 entries helpful for the design of future NGS-based marker projects. All entries of S. scabrum were found to be hexaploid, whereas entries of S. villosum were tetraploid with a haploid genome size of all samples slightly varying by approximately 1 pg. Finally, we also determined pollen viability parameters, which differed significantly between the two species but did not correlate to any of the other parameters measured.
KW - Genome size
KW - Pollen viability
KW - Polymorphism
KW - S. scabrum
KW - S. villosum
UR - http://www.scopus.com/inward/record.url?scp=85043264380&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2018.03.003
DO - 10.1016/j.scienta.2018.03.003
M3 - Article
AN - SCOPUS:85043264380
VL - 235
SP - 152
EP - 159
JO - Scientia horticulturae
JF - Scientia horticulturae
SN - 0304-4238
ER -