TY - JOUR

T1 - Development of living cell microarrays using non-contactmicropipette printing

AU - Jonczyk, Rebecca

AU - Timur, Suna

AU - Scheper, Thomas

AU - Stahl, Frank

N1 - Funding information: This work was supported by Federal Ministry of Education and Research (BMBF) and by Niedersächsische Krebsgesellschaft e.V . We are grateful to Dr. Antonina Lavrentieva and Dr. Johanna Walter for her critical reading of the manuscript. We further thank Paul Maschhoff for proofreading of the manuscript.

PY - 2015/11/19

Y1 - 2015/11/19

N2 - During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.

AB - During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.

KW - Living mammalian cell

KW - Microarray technology

KW - Piezoelectric nanoprinter

UR - http://www.scopus.com/inward/record.url?scp=84949267070&partnerID=8YFLogxK

U2 - 10.1016/j.jbiotec.2015.11.013

DO - 10.1016/j.jbiotec.2015.11.013

M3 - Article

C2 - 26603124

AN - SCOPUS:84949267070

VL - 217

SP - 109

EP - 111

JO - Journal of biotechnology

JF - Journal of biotechnology

SN - 0168-1656

ER -